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Journal of Plant Biology

, Volume 43, Issue 1, pp 48–55 | Cite as

Differential expression of the potato proteinase inhibitor II promoter in transgenic tobacco

  • Su Jin Kim
  • Mi -Yeon Lee
  • Gynheung An
  • Seong -Ryong Kim
Article

Abstract

We studied temporal and spatial expression patterns of the potato proteinase inhibitor II (PI-II) promoter, using transgenic tobacco (Nkotiana tabacum L cv. Xanthi) plants that carried a fusion between the PI-II promoter and the chloramphenicol acetyltransferase (cat) gene. Pl-ll promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the Pl-ll promoter. We used several environmental stimuli to examine the induction of the Pl-ll promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the Pl-ll gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the Pl-ll promoter. A field strength of 0.75 kV/cm and 400 μF capacitance were optimal electroporation conditions for our transient assay.

Keywords

chloramphenicol acetyltransferase electroporation promoter proteinase inhibitor transgenic tobacco 

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Copyright information

© The Botanical Society of Korea 2000

Authors and Affiliations

  • Su Jin Kim
    • 1
  • Mi -Yeon Lee
    • 1
  • Gynheung An
    • 2
  • Seong -Ryong Kim
    • 1
  1. 1.Department of Life ScienceSogang UniversitySeoulKorea
  2. 2.Department of Life Science and Division of Molecular Life SciencePohang University of Science and TechnologyPohangKorea

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