Brassinolide influences the regeneration of adventitious shoots from cultured leaf discs of tobacco

  • Song-Lim Kim
  • Yew Lee
  • Seung-Hyeon Lee
  • Soo-Hwan Kim
  • Tae-Jin Han
  • Seong-Ki Kim


When several concentrations of brassinolide (BL) were added to a shoot induction medium (SIM) that contained only BA, redifferentiation of adventitious shoots from tobacco leaf discs was unaffected at low BL levels (10-10~10-8 M), but was inhibited at higher concentrations. In comparison, when BL was applied without BA, only cell expansion occurred and no shoots formed. The determination time for shoot formation was shortened at low BL concentrations, but their formation was postponed (i.e., time was lengthened) at higher concentrations. Elongation of shoots incubated for 30 d was unaffected at low BL concentrations, but was inhibited as that amount increased.NTH1, a tobacco homeobox gene that is expressed in the central zone of the tobacco shoot apex, showed greater expression levels in the SIM over time, and its expression was stronger in media treated with low concentrations of BL compared with the SIM control at the same time point. Expression ofNTH1 was postponed at higher BL concentrations. In conclusion, at low concentrations, brassinolide has no effect on shoot formation. However, it inhibits their formation at high concentrations when cytokinin is included in the media.


Brassinosteroid Cytokinin NTH1 shoot regeneration tobacco 



Benzyl adenine






Nicotiana tabacum homeobox 1


Shoot apical meristem


Shoot induction medium

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Copyright information

© The Botanical Society of Korea 2008

Authors and Affiliations

  1. 1.Department of Life ScienceHallym UniversityChuncheonKorea
  2. 2.Department of Life ScienceChung-Ang UniversitySeoulKorea
  3. 3.Division of Biological Sciences and BiotechnologyYonsei UniversityWonjuKorea

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