Journal of Plant Biology

, Volume 50, Issue 3, pp 336–343 | Cite as

CbLEA, a NovelLEA Gene fromChorispora bungeana, Confers Cold Tolerance in Transgenic Tobacco

  • Hua Zhang
  • Rui Xia Zhou
  • Li Jing Zhang
  • Ruo Yu Wang
  • Li Zhe An


A novel late embryogenesis abundant (LEA) gene (AY804193), namedCbLEA, has now been isolated fromChorispora bungeana. This rare alpine subnival plant can survive sudden snowstorms and low temperatures. The full-lengthCbLEA is 842 bp, with an open reading frame encoding 169 ami no acids. The putative molecular weight ofCbLEA protein is 17.9 kDa, with an estimatedpl of 6.45. To investigate the functioning of thisCbLEA protein in cold-stress tolerance,CbLEA was introduced into tobacco under the control of the CaMV35S promoter. Second-generation (R1) transgenic tobacco plants exhibited significantly increased tolerance to cold. These transgenics maintained lower malondialdehyde (MDA) contents and electrolyte leakage (EL) but their relative water content (RWC) was significantly higher compared with non-transgenic plants under chilling stress. Further experimental results showed that non-transgenic plants had severe freezing damage after exposure to -2°C for 1 h, whereas the transgenics suffered only slight injury under the same conditions. Moreover, survival was longer in the latter genotype at that temperature. The extent of increased cold tolerance was positive correlated with the level ofCbLEA protein accumulation, and was also reflected by the delayed development of damage symptoms. This indicates thatCbLEA is an excellent stress tolerance gene, and holds considerable potential as a new molecular tool for engineering improved plant genetics.


chilling stress Chorispora bungeana cold tolerance freezing stress LEA gene transgenic tobacco 


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Copyright information

© The Botanical Society of Korea 2007

Authors and Affiliations

  1. 1.Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life SciencesLanzhou UniversityLanzhouChina
  2. 2.College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhouChina
  3. 3.Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of SciencesLanzhouChina

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