Journal of Plant Biology

, Volume 47, Issue 2, pp 133–141 | Cite as

Characterization of a cold-responsive gene,OsPTR1, isolated from the screening of β-Glucuronidase (GUS) gene-trapped rice

  • Ji-Youn Kim
  • Sang-Choon Lee
  • Ki-Hong Jung
  • Gynheung An
  • Seong-Ryong Kim


T-DNA gene trap populations have been successfully used to isolate stress responsive genes by probing GUS expression after stress treatment. Here, we have identified cold responsive GUS expression in rice from Line 1C-118-30, where T-DNA was inserted at the 3rd exon ofOsPTR1. This gene encodes a putative peptide transporter sharing 40% homology withArabidopsis AtPTR2 and barley HvPTR. Northern analysis ofOsPTR1 confirmed that OsPTRi is inducible by cold stress, especially in the shoot but not in the root, thereby indicating an organ-specific response. Transcript was also induced by salt, and water stresses. Interestingly,OsPTR1 mRNA was highly expressed in theUBI::CBF1/DREB1b rice, indicating thatOsPTR1 may be regulated by the CBF/DREB stress signaling pathway.


cold gene trap peptide transporter rice stress 


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Copyright information

© The Botanical Society of Korea 2004

Authors and Affiliations

  • Ji-Youn Kim
    • 1
  • Sang-Choon Lee
    • 1
  • Ki-Hong Jung
    • 2
  • Gynheung An
    • 2
  • Seong-Ryong Kim
    • 1
  1. 1.Department of Life ScienceSogang UniversitySeoulKorea
  2. 2.National Research Laboratory of Plant Functional Genomics, Department of Life SciencePohang University of Science and TechnologyPohangKorea

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