Characterization of a cold-responsive gene,OsPTR1, isolated from the screening of β-Glucuronidase (GUS) gene-trapped rice
T-DNA gene trap populations have been successfully used to isolate stress responsive genes by probing GUS expression after stress treatment. Here, we have identified cold responsive GUS expression in rice from Line 1C-118-30, where T-DNA was inserted at the 3rd exon ofOsPTR1. This gene encodes a putative peptide transporter sharing 40% homology withArabidopsis AtPTR2 and barley HvPTR. Northern analysis ofOsPTR1 confirmed that OsPTRi is inducible by cold stress, especially in the shoot but not in the root, thereby indicating an organ-specific response. Transcript was also induced by salt, and water stresses. Interestingly,OsPTR1 mRNA was highly expressed in theUBI::CBF1/DREB1b rice, indicating thatOsPTR1 may be regulated by the CBF/DREB stress signaling pathway.
Keywordscold gene trap peptide transporter rice stress
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