Journal of Plant Biology

, 50:44 | Cite as

Transgenic tobacco expressing a ring domain-containing protein ofCapsicum annuum confers improved cold tolerance

  • Kyung-A Kim
  • Eul-Won Hwang
  • Soo-Chul Park
  • Mi-Jeong Jeong
  • Myung-Ok Byun
  • Hawk-Bin Kwon


Zinc finger proteins function in plant tolerances to stresses from cold, dehydration, and salt. To determine the mechanisms for those underlying defenses, we previously used cDNA microarrays and northern blot analysis to identify a gene for the ring zinc finger protein (RDCP1) from hot pepper (Capsicum annuum). In that study, we showed that theRDCP1 gene was strongly induced by cold stress and, to a lesser degree, by ABA and high salt Here, we have used a Ti-plasmid andAgrobacterium- mediated transformation to engineerRDCP1 under the control of the CaMV35S promoter for constitutive expression in tobacco. The resultant RDCP1 transgenic plants exhibit significantly increased tolerance to low temperatures. Moreover, some of those transgenics have greater drought tolerance. In addition, none of the RDCP1 transgenic plants show any visible alterations from the wild phenotype. These current results demonstrate the biological role of RDCP1 in conferring stress tolerance.


Capsicum annuum cold tolerance ring domain-containing protein transgenic tobacco 

Literature Cited

  1. Bohnert HJ, Ajoubi P, Borchert C, Bressan RA, Burnap RL, Cushman JC, Cushman MA, Deyholos M, Fischer R, Galbraith DW, Hasegawa PM, Jenks M, Kawasaki S, Koiwa H, Kore-eda S, Lee BH, Michalowski CB, Misawa E, Nomura M, Ozturk N, Postier B, Prade R, Song CP, Tanaka Y, Wang H, Zhu JK (2001) A genomics approach towards salt stress tolerance. Plant Physiol Biochem39: 295–311CrossRefGoogle Scholar
  2. Cheong YH, Chang HS, Gupta R, Wang X, Zhu T, Luan S (2002) Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses inArabidopsis. Plant Physiol129: 661–677PubMedCrossRefGoogle Scholar
  3. Cho SK, Kim WT (2004) Genbank database submission. Genbank Accession # AY513612Google Scholar
  4. Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2002) Plant cellular and molecular response to high salinity. Annu Rev Plant Physiol Plant Mol Biol51: 463–499CrossRefGoogle Scholar
  5. Hershko A, Ciechanover A (1998) The ubiquitin system. Annu Rev Biochem67: 425–479PubMedCrossRefGoogle Scholar
  6. Holmberg N, Bulow L (1998) Improving stress tolerance in plants by gene transfer. Trends Plant Sci3–2: 61–66CrossRefGoogle Scholar
  7. Huang J, Wang JF, Wang QH, Zhang HS (2005) Identification of a rice zinc finger protein whose expression is transiently induced by drought, cold but not by salinity and abscisic acid. DNA Seq16: 130–136PubMedCrossRefGoogle Scholar
  8. Hwang EW, Kim KA, Park SC, Jeong MJ, Byun MO, Kwon HB (2005) Expression profiles of hot pepper (Capsicum annuum) genes under cold stress conditions. J Biosci30: 657–667PubMedCrossRefGoogle Scholar
  9. Joazeiro CAP;Weissman AM (2000) RING finger proteins: Mediators of ubiquitin ligase activity. Cell102: 549–552PubMedCrossRefGoogle Scholar
  10. Kim JC, Lee SH, Cheng YH, Yoo CM, Lee SI, Chun HJ, Yun DJ, HongJC, Lee SY, Lim CO, Cho MJ (2001) A novel cold inducible zinc-finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants. Plant J25: 247–259PubMedCrossRefGoogle Scholar
  11. Kosarev P, Mayer KF, Hardtke CS (2002) Evaluation and classification of RING-finger domains encoded by theArabidopsis genome. Genome Biol3: 0016.1–0016.12CrossRefGoogle Scholar
  12. Kreps JA, Wu Y, Chang HS, Zhu T, Wang X, Harper (2002) Transcriptome changes forArabidopsis in response to salt, osmotic and cold stress. Plant Physiol130: 2129–2141PubMedCrossRefGoogle Scholar
  13. Kwon HB, Park SC, Peng HF;Goodman HM, Dewdney J, Shih MC (1994) Identification of light responsive region of the nuclear gene encoding the B subunit of chloroplast glyceraidehyde 3-phosphate dehydrogenase fromArabidopsis thaliana. Plant Physiol105: 357–367PubMedCrossRefGoogle Scholar
  14. Mukhopadhyay A, Vij S, Tyagi AK (2004) Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Natl Acad Sci USA101: 6309–6314PubMedCrossRefGoogle Scholar
  15. Qiang L, Nanming Z, Yamaguchi-Shinozaki K, Shinozaki K (2000) Regulatory role of DREB transcription factors in plant drought, salt and cold tolerance. Chinese Sci Bull45: 970–975CrossRefGoogle Scholar
  16. Roh KH, Shin KS, Lee YH, Seo SC, Park HG, Daniell H, Lee SB (2006) Accumulation of sweet protein monellin is regulated by the psbA 5’LJTR in tobacco chloroplasts. J Plant Biol49: 34–43CrossRefGoogle Scholar
  17. Sahin-Cevik M, Moore GA (2006) Identification and expression analysis of cold-regulated genes from the cold-hardy Citrus relativePoncirus trifoliata (L.) Raf. Plant Mol Biol62: 83–97PubMedCrossRefGoogle Scholar
  18. Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K (2004)Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiol136: 2734–2746PubMedCrossRefGoogle Scholar
  19. Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM (2000) Coordinated plant defense responses inArabidopsis revealed by microarray analysis. Proc Natl Acad Sci USA97: 11655–11660PubMedCrossRefGoogle Scholar
  20. Seki M, Ishida j, Narusaka M, Fujita M, Nanjo T, Umezawa T, Kamiya A, Nakajima M, Enju A, Sakurai T, Satou M, Akiyama K, Yamaguchi-Shinozaki K, Carninci P, Kawai J, Hayashizaki Y, Shinozaki K (2002) Monitoring the expression pattern of around 7,000Arabidopsis genes under ABA treatments using a fulllength cDNA microarray. Funct Integr Genomics2: 282–291PubMedCrossRefGoogle Scholar
  21. Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K (2001) Monitoring the expression pattern of 1300Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell13: 61–72PubMedCrossRefGoogle Scholar
  22. Shinozaki K, Yamaguchi-Shinozaki K (1997) Gene expression and signal transduction in water stress response. Plant Physiol115: 327–334PubMedCrossRefGoogle Scholar
  23. Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol6: 410–417PubMedCrossRefGoogle Scholar
  24. Sugano S, Kaminaka H, Rybka Z, Catala R, Salinas J, Matsui K, Ohme-Takagi M, Takatsuji H (2003) Stress-responsive zinc finger gene ZPT2-3 plays a role in drought tolerance in petunia. Plant J36: 830–841PubMedCrossRefGoogle Scholar
  25. Thomashow MF (1999) Plant cold acclimation: Freezing tolerance genes and regulatory mechanism. Annu Rev Plant Physiol Plant Mol Biol50: 571–599PubMedCrossRefGoogle Scholar
  26. Xiong L, Schumaker KS, Zhu JK (2002) Cell signaling during cold, drought, and salt stress. Plant Cell14: S165–183PubMedCrossRefGoogle Scholar
  27. Xiong L, Zhu JK (2002) Molecular and genetic aspect of plant responses to osmotic stresses. Plant Cell Environ25: 131–139PubMedCrossRefGoogle Scholar
  28. Zhu JK (2001a) Cell signaling under salt, water and cold stresses. Curr Opin Biotechnol9: 214 -219Google Scholar
  29. Zhu JK (2001 b) Plant salt tolerance. Trends Plant Sci6: 66–71PubMedCrossRefGoogle Scholar
  30. Zhu T, Provart NJ (2003) Transcriptional responses to low temperature and their regulation inArabidopsis. Can J Bot Rev Can Bot81: 1168–1174CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2007

Authors and Affiliations

  • Kyung-A Kim
    • 1
  • Eul-Won Hwang
    • 1
  • Soo-Chul Park
    • 2
  • Mi-Jeong Jeong
    • 2
  • Myung-Ok Byun
    • 2
  • Hawk-Bin Kwon
    • 1
  1. 1.Division of Applied Biological SciencesSunmoon UniversityAsanKorea
  2. 2.Abiotic and Biotic Stress TeamNational Institute of Agricultural BiotechnologySuwonKorea

Personalised recommendations