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Journal of Plant Biology

, 50:24 | Cite as

Direct and indirect regeneration of plants from internodal and leaf expiants ofHypencum bupleuroides gris

  • Cüneyt çirak
  • Ali Kemal Ayan
  • Kudret KevseroĞlu
Article

Abstract

Species of the genusHypencum are of considerable interest worldwide because of their medicinal properties.In- vitro culture is a useful tool for both multiplication of the genus and studying its economically important secondary metabolites. Here, we present an effectivein- vitro propagation method forH. bupleuroides. Leaf and internodal expiants excised from 9-week-old,in vitro-germinated seedlings were cultured on a Murashige and Skoog (MS) medium supplemented with benzyladenine (BA; 1.0 or 0.1 mg L-1) and 2,4-dichlorophenoxyacetic acid (2,4-D; 1.0 or 0.1 mg L1). Depending on the BA and 2,4-D combination used, these cultures produced adventitious shoot buds directly on the surfaces of both types of explants as well as excessive calli. Numerous shoots were obtained when the calli from both expiant types were cultured on an MS medium supplemented with 2 mg L-1 BA. Internodal expiants were more responsive than leaf tissues to direct and indirect plant regeneration. After shoots that regenerated from either the calli or the expiant surface were excised, rooting was best on an MS medium lacking any growth hormones. These rooted plants were then acclimatized under greenhouse conditions, and 90% of regenerants had survived. Ours is the first report ofin- vitro plant regeneration fromH. bupleuroides.

Keywords

callus induction Hypencum bupleuroides plant growth regulators plant regeneration rooting shoot induction 

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Copyright information

© The Botanical Society of Korea 2007

Authors and Affiliations

  • Cüneyt çirak
    • 1
  • Ali Kemal Ayan
    • 2
  • Kudret KevseroĞlu
    • 1
  1. 1.Faculty of Agriculture, Department of AgronomyUniversity of Ondokuz MayisSamsun/Turkey
  2. 2.the High School of Profession of BafraUniversity of Ondokuz MayisSamsun/Turkey

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