Journal of Plant Biology

, Volume 48, Issue 4, pp 422–428 | Cite as

Biochemical characterization of anArabidopsis glucosyltransferase with high activity toward Jasmonic acid

  • Jong Tae Song


Biochemical characterization of the recombinant gene products from theArabidopsis glucosyltransferase multigene family has identified one enzyme with high activity toward the plant cellular regulator jasmonic acid (JA). The protein, AtJGT1 (UDP-glucose:JA glucosyltransferase), also has significant activities with other substrates, such as dihydrojasmonicacid, indole-3-acetic acid (IAA), indole-3-propionic acid, and indole-3-butyric acid. TheK M values of AtJGT1 for JA or IAA are similar to those of anArabidopsis IAA glucosyltransferase UGT84B1 previously reported. Northern blot analysis showed thatAtJGTI is highly expressed in the leaves, but only slightly detectable in the roots, stems, and inflorescences. This study describes the first biochemical analysis of a recombinant glucosyltransferase with JA activity, and provides the foundation for future genetic approaches to understanding the role of JA-glucose inArabidopsis.


glucosyltransferase hormone homeostasis indole-3-acetic acid jasmonic acid JA conjugate 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem72: 248–254PubMedCrossRefGoogle Scholar
  2. Cohen JD, Bandurski RS (1982) Chemistry and physiology of the bound auxins. Annu Rev Plant Physiol33: 403–430CrossRefGoogle Scholar
  3. Epstein E, Ludwig-Müller J (1993) lndole-3-butyric acid in plants: Occurrence, synthesis, metabolism, and transport. Physiol Plant88: 382–389CrossRefGoogle Scholar
  4. Guan KL, Dixon JE (1991) Eukaryotic proteins expressed inEscherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal Biochem192: 262–267PubMedCrossRefGoogle Scholar
  5. Jackson RG, Kowalczyk M, Li Y, Higgins G, Ross J, Sandberg G, Bowles DJ (2002) Over expression of anArabidopsis gene encoding a glucosyltransferase of indole-3-acetic acid: Phenotypic characterization of transgenic lines. Plant J32: 573–583PubMedCrossRefGoogle Scholar
  6. Jackson RG, Lim EK, Li Y, Kowalczyk M, Sandberg G, Hoggett J, Ashford DA, Bowles DJ (2001) Identification and biochemical characterization of anArabidopsis indole-3-acetic acid glucosyltransferase. J Biol Chem276: 4350–4356PubMedCrossRefGoogle Scholar
  7. Ji JH, Lee JS, Lee JS, Kim WT (1995) Effects of methyl jasmonate on ethylene production in tomato (Lycopersicon esculentum Mill.) hypocotyl segments and fruits. J Plant Biol38: 235–242Google Scholar
  8. Kroczek RA, Siebert E (1990) Optimization of northern analysis by vacuum-blotting, RNA transfer, visualization and ultraviolet fixation. Anal Biochem184: 90–95PubMedCrossRefGoogle Scholar
  9. Lee H, Raskin I (1999) Purification, cloning, and expression of a pathogen inducible UDP glucose: Salicylic acid glucosyltransferase from tobacco. J Biol Chem274: 36637–36642PubMedCrossRefGoogle Scholar
  10. Li Y, Baldauf S, Lim E-K, Bowles DJ (2001) Phylogenetic analysis of the UDP glycosyltransferase multigene family ofArabidopsis thaliana. J Biol Chem276: 4338–4343PubMedCrossRefGoogle Scholar
  11. Normanly J (1997) Auxin metabolism. Physiol Plant100: 431–442CrossRefGoogle Scholar
  12. Sambrook J, Fritsch, EF, Maniatis T (1989) Molecular Cloning, A Laboratory Manual, Ed 2. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  13. Sembdner G, Atzorn R, Schneider G (1994) Plant hormone conjugation. Plant Mol Biol26: 1459–1481PubMedCrossRefGoogle Scholar
  14. Sembdner G, Parthier B (1993) The biochemistry and the physiological molecular actions of jasmonates. Annu Rev Plant Physiol Plant Mol Biol44: 569–589CrossRefGoogle Scholar
  15. Seo HS, Song JT, Cheong JJ, Lee YH, Lee YW, Hwang I, Lee JS, Choi YD (2001) Jasmonic acid carboxyl methyltransferase: A key enzyme for jasmonate-regulated plant responses. Proc Natl Acad Sci USA98: 4788–4793PubMedCrossRefGoogle Scholar
  16. Song JT, Seo HS, Song SI, Lee JS, Choi YD (2000) NTR1 encodes a floral nectary-specific gene inBrassica campestris L. ssp.pekinensis. Plant Mol Biol42: 647–655CrossRefGoogle Scholar
  17. Staswick PE, Tiryaki I (2004) The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine inArabidopsis. Plant Cell16: 2117–2127PubMedCrossRefGoogle Scholar
  18. Staswick PE, Tiryaki I, Rowe ML (2002) Jasmonate response locus JAR1 and several relatedArabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation. Plant Cell14: 1405–1415PubMedCrossRefGoogle Scholar
  19. Szerszen JB, Szczyglowski K, Bandurski RS (1994)iaglu, a gene from Zeamays involved in conjugation of growth hormone indole-3-acetic acid. Science265: 1699–1701PubMedCrossRefGoogle Scholar
  20. Tarn YY, Epstein E, Normanly J (2000) Characterization of auxin conjugates inArabidopsis: Low steady-state levels of indole-3-acetyl-aspartate, indole-3-acetyl-glutamate, and indole-3-acetyl-glucose. Plant Physiol123: 589–596CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2005

Authors and Affiliations

  1. 1.Division of Plant BiosciencesKyungpook National UniversityDaeguKorea

Personalised recommendations