Characterization of a drought-responsive gene,OsTPS1, identified by the T-DNA Gene-Trap system in rice
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Trehalose-6-phosphate synthase (TPS) is a key enzyme for trehalose biosynthesis. To understand the function of theTPS gene, we identified the T-DNA insertion in aTPS gene of rice. Line 1C-071-05 had T-DNA inserted at the second intron ofOsTPSI. Its deduced OsTPSI protein shared 52% homology withArabidopsis TPS. Reverse transcriptase-PCR analysis ofOsTPS1 showed thatOsTPS1 is inducible by drought, salt, cold, and ABA. Leaves ofOsTPS1 knockout (KO) plants were more sensitive to drought or cold stress than were the wild types. Furthermore, transgenic rice ofUBI::CBF1 had high expressionOsTPS1 mRNA, suggesting thatOsTPSI is regulated by the CBF/DREB transcription factor. Therefore, we propose thatOsTPSI plays an important role during the abiotic stress response.
Keywordsdrought rice T-DNA trehalose-6-phosphate synthase
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