Low expression profiles of heat stress-related genes inCapsicum annuum
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A cDNA library was constructed for hot pepper plants that had been heat-shock-treated. We used a modified differential screening method, double negative screening, to isolate 500 cDNA clones that represented genes with low expression levels under conditions of high-temperature stress. Of those 500 clones, 200 were randomly selected for single-read sequencing from the 5′ ends. After annotation with Blastx, the sequence was applied to InterProScan to scan for functional motifs of proteins. Among the cDNA clones analyzed, about 41% of the ESTs could not be functionally classified. However, of those that could be, the largest portion of the ESTs (15%) were assigned to the category of cell rescue and defense; genes involved in cell cycle/DNA processing constituted the smallest group, comprising 1% of the ESTs. Genes related to energy and protein fates constituted the second (10%) and third (9%) largest groups, respectively. Finally, 3% of the ESTs were assigned to transcription, and 2% to signal transduction. The high portion of unclassified ESTs probably resulted from the screening method, which was designed for low-expression messages. Likewise, the high number of ESTs for cell rescue and defense suggests that many genes with low levels of expression are associated with the stress response.
KeywordscDNA library double negative screening high-temperature stress hot pepper (Capsicum annuum)
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