Effect of polyamines on adventitious root formation from Tobacco (Nicotiana tabaccum) leaf segments
- 147 Downloads
To elucidate the effect of polyamines on adventitious root formation, we investigated the relationship between the frequency of adventitious root formation and the endogenous content of free polyamines in tobacco leaf segments which had been treated with polyamine biosynthesis inhibitors and polyamines. Adventitious root formation was inhibited in rooting medium (10 μM IAA) with methylglyoxal-bis(guanylhydrazone) (MGBG) or cyclohexylamine (CHA), and promoted with spermidine and putrescine. Treatment with high IAA (100 μM) medium plus CHA or MGBG promoted rooting up reversion of the rooting inhibition than the one treated with high IAA concentration alone. Spermidine promoted adventitious root numbers on low IAA (1 μM) medium when applied during culture period. The rooting inductive phase (in the presence of IAA) was determined by periodical transfer of leaf segments from IAA-containing medium to IAA free medium, and by changing polyamine contents, to be inductive phase. Putrescine and spermidine were accumulated to a maximum during the inductive phase. Therefore, the results point out the involvement of polyamines in inductive phase of adventitious root formation in tobacco leaf segments.
Keywordsadventitious root spermidine spermine MGBG CHA
Unable to display preview. Download preview PDF.
- Bagni N. and P. Torrigiani. 1992. Polyamines: a new class of growth substances. In progress in plant growth regulation, Karssen CM., Van Loon L.C. and Vreugdenhil D., ed., Klumer Academic Pub., Dordrecht, The Netherland, 264–275.Google Scholar
- Evens, P.T. and R.L. Malmberg. 1989. Do polyamines have roles in plant development.Annu. Rev. Plant Physiol. Plant Mol. Biol.40: 235–269.Google Scholar
- Goren R., N. Palavan, H. Flores and A.W. Galston. 1982. Changes in poly amine titer in etiolated pea seedlings following red-light treatment.Plant Cell Physiol.23: 19–26.Google Scholar
- Han, T.J., D.W. Lee and S.H. Lee. 1994. Effects of poly-amine inhibitors and polyamines adventitious root formation from soybean cotyledons.Korean J. Plant Tissue Culture.21: 106–110.Google Scholar
- Han, T.J. and H.I. Jo. 1994. Effects of cyclohexylamine and polymaines on the adventitious root formation from soybean cotyledons. KoreanJ.Plant Tissue Culture.21: 207–213.Google Scholar
- Han, T.J., M.G. Jeon and H.I. Jo. 1996 Effect of polyamines on adventitious shoot formation on tobacco (Nicotiana tabaccum) leaf segments.Korean J. Plant Tissue Culture23: 45–50.Google Scholar
- Jarvis B.C.P., R.M. Shannon and S. Yasmin. 1983 Involvement of polyamines with adventitious root development in stem cuttings of mung bean.Plant Cell Physiol.24: 677–683.Google Scholar
- Santos, M., I. Claparols and J.M. Torne. 1993. Effect of exogenous arginine, ornithine, methionine and r-amino butyric acid on maize (Zea mays L.) embryogenesis and polyamine content.J. Plant Physiol.142: 74–80.Google Scholar
- Tanimoto, S., Y. Matsubara and N. Ishioka. 1994. Significance of spermidine in the initiation of adventitious buds in stem segments ofTorenia.Plant Cell Physiol.35: 1071–1077.Google Scholar
- Tiburcio, A.F., R. Kaur-Sawhney and A.W. Galston. 1988. Polyamine biosynthesis during vegetative and floral bud differentiation in thin layer tobacco tissue cultures.Plant cell Physiol.29: 1241–1249.Google Scholar
- Torrigiani, P., M.M. Altmura, S. Scaramagli, F. Capitani, G. Falasca and N. Bagini. 1993. Regulation of rhizogenesis by polyamines in tobacco thin layers.J. Plant Physiol.142: 81–87.Google Scholar