Journal of Plant Biology

, Volume 46, Issue 3, pp 173–180 | Cite as

In-vitro regeneration of olive tree by somatic embryogenesis

  • El Bahri Trabelsi
  • Sadok Bouzid
  • Monji Bouzid
  • Nedra Elloumi
  • Zina Belfeleh
  • Abdallah Benabdallah
  • Rachida Ghezel


We induced somatic embryogenesis from the cotyledon segments ofOlea europaea (L) cvs. ‘Chetoui’, ‘Chemleli’, and ‘Arbequina’. Calli were established from all three cultvars on OMc media supplemented with IBA and 2i-R The greatest success was obtained with media that contained zero or low concentrations of growth regulators. High levels of hormones (i.e.,>0.5 mgL-1 IBA and 2i-P) inhibited embryogenesis. Embryos at different maturation stages were observed with continuously proliferating secondary embryogenesis. Abnormally shaped embryos and teratoma were also noted. Four weeks was the optimal incubation period for inducing embryogenesis on the auxin-containing medium. In addition, 30 to 40 gL-1 sucrose was more effective than glucose in stimulating the growth and maturation of somatic embryos. Embryogeic efficiency was also higher when multivariate combinations of nitrogen sources (inorganic and organic nitrogen forms) were used. The plantlets that were derived from our germinating somatic embryos were similar to those obtained from axillary buds.


Olea europaea L. regeneration somatic embryogenesis 



cultivar ‘Arbequina’


cultivar ‘Chetoui’


cultivar ‘Chemleli’


lndole-3-butyric acid


isopentenyl adenine


Olive medium (Rugini, 1984)


Olive modified medium (Canas and Benbadis, 1988)


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Copyright information

© The Botanical Society of Korea 2003

Authors and Affiliations

  • El Bahri Trabelsi
    • 1
    • 2
  • Sadok Bouzid
    • 1
  • Monji Bouzid
    • 2
  • Nedra Elloumi
    • 3
  • Zina Belfeleh
    • 3
  • Abdallah Benabdallah
    • 3
  • Rachida Ghezel
    • 3
  1. 1.Laboratory of Plant Biology and BiotechnologyFaculty of Sciences of Tunis Campus UniversitaireTunisTunisia
  2. 2.lnstitut de lolivier de TunisieRue Hedi KarrayArianaTunisia
  3. 3.National Institute of Agronomic Research of TunisiaRue Hedi KarrayArianaTunisia

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