Journal of Plant Biology

, Volume 46, Issue 2, pp 130–133 | Cite as

Identification of a flower-specific cDNA,RsPCP1, encoding putative pollen coat protein from radish

  • Yung Geun Yoo
  • Sang -Choon Lee
  • Seong -Ryong Kim


To better understand the molecular control of floral development, we identified a flower-specific cDNA,RsPCPI, from Korean radish (Raphanus sativus). Based on nucleotide sequence analysis, this clone contains an open reading frame of 65 amino acids and shares 91% identity with a pollen coat protein from cabbage (Brassica oleracea). Southern analysis revealed thatRsPCPI is present as a single-copy gene or a member of a small gene family in the radish genome. BecauseRsPCPI mRNA was present exclusively in mature floral buds but not in young floral buds or in vegetative tissues, we propose that this gene is anther-specific.


flower-specific pollen coat protein radish 


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Literature Cited

  1. Davis LG, Dibner MD, Batty JF (1986) Basic Methods in Molecular Biology. Elsevier, New YorkGoogle Scholar
  2. Doughty J, Hedderson F, McCubbin A, Dickinson H (1993) Interaction between a coating-borne peptide of theBrassica pollen grain and stigmatic S (Self-lncompat-ibility)-locus-specific glycoproteins. Proc Nat Acad Sci USA90: 467–471PubMedCrossRefGoogle Scholar
  3. Foster GD, Robinson SW, Blundell RP, Roberts MR, Hodge R, Draper J, Scott RJ (1992) ABrassica napus mRNA encoding a protein homologous to phospholipid transfer proteins is expressed specifically in the tapetum and developing microspores. Plant Sci84: 187–192CrossRefGoogle Scholar
  4. Goldberg RB, Beals TP, Sanders PM (1993) Anther development: Basic principles and practical applications. Plant Cell5: 1217–1229PubMedCrossRefGoogle Scholar
  5. Heslop-Harrison Y (2000) Control gates and micro-ecology: The pollen-stigma interaction in perspective. Ann Bot85: 5–13CrossRefGoogle Scholar
  6. Jeon JS, Chung YY, Lee S, Yi GH, Oh BG, An G (1999) Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza saliva L.). Plant Mol Biol39: 35–44PubMedCrossRefGoogle Scholar
  7. Kachroo A, Nasrallah ME, Nasrallah JB (2002) Self-incompatibility in the Brassicaceae: receptor-ligand signaling and cell-to-cell communication. Plant Cell14: S227-S238PubMedGoogle Scholar
  8. Kamalay JC, Goldberg RB (1980) Regulation of structural gene expression in tobacco. Cell19: 934–946CrossRefGoogle Scholar
  9. Kim HU, Hsieh K, Ratnayake C, Huang AH (2002) A novel group of oleosins is present inside the pollen of Arabidopsis. J Biol Chem277: 22677–22684PubMedCrossRefGoogle Scholar
  10. Kim SR, An G (1996) Isolation and characterization of a pollen-specific cDNA clone from Easter lily. J Plant Biol39: 197–202Google Scholar
  11. Kim SR, Kim Y, An G (1993) Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor. Plant Mol Biol21: 39–45PubMedCrossRefGoogle Scholar
  12. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein metabolism. J Mol Biol157: 105–132PubMedCrossRefGoogle Scholar
  13. Lorenzo O, Calvo A, Nicolas C, Nicolas G, Rodriguez D (1999) Isolation, sequence analysis and expression of a LEA protein ofFagus sylvatica L. seeds (Accession No AJ 130888) (PGR99-017). Plant Physiol119: 805CrossRefGoogle Scholar
  14. Mariani C, de Beuckeleer M, Truettner J, Leemans J, Goldberg RB (1990) Induction of male sterility in plants by a chimaeric ribonuclease gene. Nature347: 737–741CrossRefGoogle Scholar
  15. Mascarenhas JP (1990) Gene activity during pollen development. Ann Rev Plant Physiol Plant Mol Biol41: 317–338CrossRefGoogle Scholar
  16. Orr W, lu B, White TC, Robert LS, Singh J (1992) Complementary DNA sequence of a low temperature-inducedBrassica napus gene with homology to theArabidopsis thaliana kin1 gene. Plant Physiol98: 1532–1534PubMedCrossRefGoogle Scholar
  17. Rubinelli P, Hu Y, Ma H (1998) Identification, sequence analysis and expression studies of novel anther-specific genes ofArabidopsis thaliana. Plant Mol Biol37: 607–619PubMedCrossRefGoogle Scholar
  18. Stanchev BS, Doughty J, Scutt CP, Dickinson H Cray RR (1996) Cloning of PCP1, a member of a family of pollen coat protein (PCP) genes fromBrassica oleracea encoding novel cysteine-rich proteins involved in pollenstigma interactions. Plant J10: 303–313PubMedCrossRefGoogle Scholar
  19. Steiner-Lange S, Unte US, Eckstein L, Yang C, Wilson ZA, Schmelzer E, Dekker K, Saedler H (2003) Disruption ofArabidopsis thaliana MYB26 results in male sterility due to non-dehiscent anthers. Plant J34: 519–528PubMedCrossRefGoogle Scholar
  20. Waiden AR, Walter C, Gardner RC (1999) Genes expressed inPinus radiata male cones include homologs to anther-specific and pathogenesis response genes. Plant Physiol121: 1103–1116CrossRefGoogle Scholar
  21. Willing RP, Bashe D, Mascarenhas JP (1988) An analysis of the quantity and diversity of messenger RNAs from pollen and shoots ofZea mays. Theor Appl Genet75: 751–753CrossRefGoogle Scholar
  22. Willing RP, Mascarenhas JP (1984) Analysis of the complexity and diversity of mRNAs from pollen and shoots ofTradescantia. Plant Physiol75: 865–868PubMedCrossRefGoogle Scholar
  23. Wu SS, Suen DF, Chang HC, Huang AH (2002) Maize tapetum xylanase is synthesized as a precursor, processed and activated by a serine protease, and deposited on the pollen. J Biol Chem277: 49055–49064PubMedCrossRefGoogle Scholar
  24. Yui R, Iketani S, Mikami T, Kubo T (2003) Antisense inhibition of mitochondrial pyruvate dehydrogenase El alpha subunit in anther tapetum causes male sterility. Plant J34: 57–66PubMedCrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2003

Authors and Affiliations

  • Yung Geun Yoo
    • 1
  • Sang -Choon Lee
    • 1
  • Seong -Ryong Kim
    • 1
  1. 1.Department of Life ScienceSogang UniversitySeoulKorea

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