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Journal of Plant Biology

, Volume 45, Issue 1, pp 37–43 | Cite as

Manifestation of a prolonged lag in the photosynthesis of heated spinach chloroplasts

  • Sung -Soo Jun
  • Young -Nam Hong
Article
  • 83 Downloads

Abstract

When the time course for CO2 fixation and O2 evolution in isolated intact spinach chloroplasts was examined, we found a prolonged lag time in the early phase of photosynthesis after heat-treatment in the dark as well as an expected time-dependent decrease in the rate during the subsequent linear phase. Because the lengthening of the lag period was generally attributed to the depletion of sugar phosphates in the chloroplasts, we tested for the possible involvement of Calvin cycle intermediates in the change of the lag phase by heat-treatment When triose phosphate was added to the heated chloroplasts, the lag time was re-shortened without the rate in the linear phase being elevated to that measured in the control. Mg-ATP or triose phosphate plus oxaloacetate (previously known as protective chemicals) prevented the lengthening of the lag time when added prior to heat-treatment. Quantification of some metabolites in the chloroplasts confirmed that heavy losses had occurred for triose phosphate, fructose-1,6-bis-phosphate, glucose-6-phosphate, and fructose-6-phosphate. However, the level of 3-phosphoglyceric acid was increased. The presence of Mg-ATP during heat-treatment alleviated the losses of those sugar phosphates. Therefore, we conclude that the decrease in sugar phosphates in the chloroplasts, as part of the negative effect from heat-treatment, is the primary cause of the lengthened lag time during the initial phase of photosynthesis.

Keywords

Heat-treatment isolated chloroplast lag period photoassimilation 

Abbreviations

Chl

chlorphyll

F6P

fructose-6-phosphate

FBP

fructose-6-biophosphate

G3P

glyceraldehyde-3-phosphate

G6P

glucose-6-phosphate

OAA

oxaloacetic acid

PGA

3-phosphoglyceric acid

Ru5P

ribulose-5-phosphate

RuBP

ribulose-1,5-bisphosphate

RSP

ribose-5-phosphate

sugar-P

sugar phosphate

triose-P

triose phosphate.

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Literature cited

  1. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase inBeta vulgaris. Plant Physiol24: 1–15PubMedCrossRefGoogle Scholar
  2. Baldry CW, Bucke C, Walker DA (1966a) Temperature and photosynthesis I. Some effects of temperature on carbon dioxide fixation by isolated chloroplasts. Biochim Biophys Acta126: 207–213PubMedCrossRefGoogle Scholar
  3. Baldry CW, Walker DA, Bucke C (1966b) Calvin-cycle intermediates in relation to induction phenomena in photosynthetic carbon dioxide fixation by isolated chloroplasts. Biochem J101: 642–646PubMedGoogle Scholar
  4. Bamberger ES, Gibbs M (1965) Effect of phosphorylated compounds and inhibitors on CO2 fixation by intact spinach chloroplasts. Plant Physiol40: 919–926PubMedCrossRefGoogle Scholar
  5. Bauer H, Senser M (1979) Photosynthesis of ivy leaves (Hederahelix L.) after heat stress. II. Activity of ribulose bisphosphate carboxylase, Hill reaction, and chloroplast ultrastructure. Z Pflanzenphysiol91: 359–369Google Scholar
  6. Berry J, Björkman O (1980) Photosynthetic response and adaptation to temperature in higher plants. Ann Rev Plant Physiol31: 491–543CrossRefGoogle Scholar
  7. Bucke C, Walker DA, Baldry CW (1966) Some effects of sugars and sugar phosphates on carbon dioxide fixation by isolated chloroplasts. Biochem J101: 636–641PubMedGoogle Scholar
  8. Chollet R, Anderson LL (1976) Regulation of ribulose-1,5-bisphosphate carboxylase-oxygenase activities by temperature pretreatment and chloroplast metabolites. Arch Biochem Biophys176: 344–351PubMedCrossRefGoogle Scholar
  9. Crafts-Brandner SJ, Law RD (2000) Effect of heat stress on the inhibition and recovery of the ribulose-1,5-bisphosphate carboxylase/oxygenase activation state. Planta212: 67–74PubMedCrossRefGoogle Scholar
  10. Czok R (1984) D-Clycerate 3-phosphate,In HU Bergmeyer, ed, Methods of Enzymatic Analysis, 3rd Ed, Vol 6. Verlag Chemie, Weinheim, pp 537–541Google Scholar
  11. Emmett JM, Walker DA (1969) Thermal uncoupling in chloroplasts. Biochim Biophys Acta180: 424–425PubMedCrossRefGoogle Scholar
  12. Emmett JM, Walker DA (1973) Thermal uncoupling in chloroplasts. Inhibition of photophosphorylation without depression of light-induced pH change. Arch Biochem Biophys157: 106–113PubMedCrossRefGoogle Scholar
  13. Feller U, Crafts-Brandner SJ, Salvucci ME (1998) Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activasemediated activation of Rubisco. Plant Physiol116: 539–546PubMedCrossRefGoogle Scholar
  14. Fu CF, Gibbs M (1988) Effects of temperature pretreatment in the dark on photosynthesis of the intact spinach chloroplast. Plant Physiol88: 207–212PubMedCrossRefGoogle Scholar
  15. Jun S-S, Kim JM, Lee CB (2001) A comparative study on the effect of chilling treatment in the light and in the dark on subsequent photosynthesis in cucumber. Aus J Plant Physiol28: 489–496Google Scholar
  16. Jun S-S, Lee CB, Hong Y-N (1994) Evidence against the involvement of stromal acidification in the heat induced inhibition of photosynthesis in isolated spinach chloroplasts. J Biochem Mol Biol27: 205–210Google Scholar
  17. Katoh S, San Pietro A (1967) Ascorbate-supported NADP photoreduction by heatedEuglena chloroplasts. Arch Biochem Biophys122: 144–152PubMedCrossRefGoogle Scholar
  18. Krause GH, Santarius KA (1975) Relative thermostability of the chloroplast envelope. Planta127: 285–299CrossRefGoogle Scholar
  19. Law RD, Crafts-Brandner SJ (1999) Inhibition and acclimation of photosynthesis to heat stress is closely correlated with activation of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol120: 173–181PubMedCrossRefGoogle Scholar
  20. Lowry OH, Passonneau JV (1972) A flexible system of enzymatic analysis. Academic Press, New York and LondonGoogle Scholar
  21. Michal G (1984) D-Glucose 6-phosphate and D-fructose 6-phosphate,In HU Bergmeyer, ed, Methods of Enzymatic Analysis, 3rd Ed, Vol 6. Verlag Chemie, Weinheim, pp 191–198Google Scholar
  22. Osterhout WJV, Haas ARC (1918) On the dynamics of photosynthesis. J Gen Physiol1: 1–16CrossRefGoogle Scholar
  23. Santarius KA (1975) Sites of heat sensitivity in chloroplasts and differential inactivation of cyclic and noncyclic photophosphorylation by heating. J Thermal Biol1: 101–107CrossRefGoogle Scholar
  24. Thomas PC, Quinn PJ, Williams WP (1986) The origin of photosystem-l-mediated stimulation in heat-stressed chloroplasts. Planta167: 133–139CrossRefGoogle Scholar
  25. Turner JF, Black CC, Gibbs M (1962) Studies on photosynthetic processes. I. The effect of light intensity on triphosphopyridine nucleotide reduction, adenosine triphosphate formation, and carbon dioxide assimilation in spinach chloroplasts. J Biol Chem237: 577–579PubMedGoogle Scholar
  26. Walker DA (1976) CO2 fixation by intact chloroplasts: photosynthetic induction and its relation to transport phenomena and control mechanism,In J Barber, ed, The Intact Chloroplast, Elsevier/North-Holland Biomedical Press, Amsterdam, pp 235–278Google Scholar
  27. Weis E (1981a) The temperature-sensitivity of dark-inactivation and light-activation of ribulose-1,5-bisphosphate carboxylase in spinach chloroplasts. FEBS Lett129: 197–200CrossRefGoogle Scholar
  28. Weis E (1981b) Reversible heat inactivation of the Calvin cycle: a possible mechanism of the temperature regulation of photosynthesis. Planta151: 33–39CrossRefGoogle Scholar
  29. Weis E (1982) Influence of light on the heat sensitivity of the photosynthetic apparatus in isolated spinach chloroplasts. Plant Physiol70: 1530–1534PubMedCrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2002

Authors and Affiliations

  1. 1.School of Biological SciencesSeoul National UniversitySeoulKorea

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