Journal of Plant Biology

, Volume 44, Issue 4, pp 193–198 | Cite as

The effect of the recombinant human interleukin-2 Gene in potato (Solanum tuberosum cv. superior)

  • Yoonkyung Park
  • Hyeonsook Cheong


To examine the effect of the T-cell growth factor (human interleukin-2), we constructed a binary vector, pSSK-1, carrying the recombinant human interleukin-2 (rhlL-2) gene, and transferred it intoAsrobacterium tumefaciens. Using this construct, we then transformed potato explants(Solanum tuberosum cv. Superior), achieving 100% regeneration of shoots on a modified MS medium. Of the putative transformed shoots, 81% rooted and were selected on 200 ms/L kanamycin. Both Southern and northern analyses verified the transformation events. An ELISA test also indicated that the rhlL-2 protein was produced from rhlL-2-transformed potatoes. To determine whether this protein was biologically active in the potato cells, we performed a biological assay using the 11.-2 dependent cell line, CTLL-2. The suspension containing extract from the transformants showed significant proliferation of the 11.-2 dependent CTLL-2 cells, whereas cells did not proliferate in the nontransformed potato. We then grew the verified rhlL-2 transgenic potatoes in soil, and compared their performance with that of nontransgenic potatoes as well as those that had been transformed with GUS. Growth rates, as calculated from plant heights, were up to 50% higher than for either the nontrans-genic or the GUS-transformed potatoes. Similar patterns were found withArabidopsis thaliana plants treated in the same manner. All of these results suggest that rhlLo2 may function as a growth factor in potato.


Arabidopsis thaliana potato (Solanum tuberosum cv. Superior) recombinant human interleukin-2 (rhlL-2) T-cell growth factor 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature Cited

  1. Bernd MR, Jens K, Hannah LC, Willmitzer L, Sonnewald U (1990) One of two different ADP-glucose pyrophos-phorylase genes from potato responds strongly to elevated levels of sucrose. MGG224: 35–42Google Scholar
  2. Bubenik J, Indrova M, Perlmann R Berzins K, Mach O, Kraml J, Toulcova A (1985) Tumour inhibitory effects of TCGF/IL-2/-containing preparations. Cancer Immunol19:57–61Google Scholar
  3. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Aced Sci USA81: 1991–1995CrossRefGoogle Scholar
  4. Cone K (1989) Yet another plant DNA reparation. Maize Genetics Newsletter, pp 63–68Google Scholar
  5. Culver JN, Lehtok, Close SM, Hilf ME, Dawson WO (1993) Genomic position effects the expression of tobacco mosaic virus movement and coat proteins genes. Proc Natl Acad Sci USA90: 2055PubMedCrossRefGoogle Scholar
  6. Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay. Quantitative assay of immunoglobulin G Immunochemistry18: 871–874Google Scholar
  7. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high spcecific activity. Anal Biochem132: 6–13PubMedCrossRefGoogle Scholar
  8. Gillis S, Ferm MM, Ou W, Smith KA (1978) T cell growth factor, parameters of production and quantitative microassay for activity. J Immuno1120: 2027–2032Google Scholar
  9. Hiatt A (1990) Antibodies produced in plants. Nature344: 469–470PubMedCrossRefGoogle Scholar
  10. Hiatt A, Caffertey R, Bowdish K (1989) Production of antibodies in transgenic plants. Nature342: 76–78PubMedCrossRefGoogle Scholar
  11. Hong CB, Jeon JH (1987) A sample procedure for RNA isolation from plants and preservation of plant material for RNA analysis. KorJ Bot30: 201–203Google Scholar
  12. Hong CB, Park SM (1997) Transcription of human interleukin-2 Gene inNicotiana tabacum driven by CaMV35S promoter. J Plant Biol40: 110–114CrossRefGoogle Scholar
  13. Horsch RB (1985) A simple and general method for transforming genes into plants. Science227: 1229–1231CrossRefGoogle Scholar
  14. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusion: ß-glucuronidase as a sensitive and versatile gene marker in higher plants. EMBO J6: 3901–3907PubMedGoogle Scholar
  15. Jeffrey SR, Corthesy B, Flanagan WM, Crabtree GR (1992) Regulation of the interleukin-2 gene. Interleukins: molecular biology and immunology. Chem Immunol Basel Karger51: 266–298CrossRefGoogle Scholar
  16. Jeyaseelan K, Chang MCM, Kon OL (1987) Genes and Proteins Manual of Selected Techniques in Molecular Biology. ICSU Press, pp 151–170Google Scholar
  17. Ma JK, Hiatt A, Hein M, Vine ND, Wang F, Stabila P, van Dolleweerd C, Mostov K, Lehner T (1995) Generation and assembly of secretory antibodies in plants. Science268: 716–719PubMedCrossRefGoogle Scholar
  18. Murashige T, Skoog F (1962) A revised medium for growth and bioassay with tobacco tissue culture. Physiol Plant15:473–497CrossRefGoogle Scholar
  19. Rosenberg SA, Lotze MT, Muul LM, Leitman S, Chang AE, Ettinghausen SE, Matory YL, Skibber JM, Shiloni E, Vetto JT (1985) Observations on the systemic administration of autologous lymphokine-activated killer cells and recombinant interleukin-2 to patients with metastatic cancer. New Engl J Med313: 1485–1492PubMedGoogle Scholar
  20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Clonirig, a Laboratory Manual 2nd ed, Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  21. Smith KA (1988) Interleukin-2: Inception, impact and implications. Science240: 1169–1175PubMedCrossRefGoogle Scholar
  22. Sree-Ramulu K (1987) Case histories of genetic variability in vitro,In Cell Culture and Somatic Cell Genetics of Plants, Academic Press, NYGoogle Scholar
  23. Stiekema W, Heidekamp F, Louwerse J, Verhoeven H, Dijkhuls P (1988) Introduction of foreign genes into potato cultivars Bintje and Desiree using an Agrobacte-riumtumefaciens binary vector. Plant Cell Rep7: 47–50CrossRefGoogle Scholar
  24. Taniguchi T, Hiroshi M, Takashi F, Chikako T, Nobukazu K, Ryota Y, Junji H (1983) Structure and expression of a cloned cDNA for human interleukin-2. Nature302: 305–310PubMedCrossRefGoogle Scholar
  25. Valvekens D, Muntagu MV, Lijsebettens MV (1988) Transformation ofArabidopsis thaliana. Proc Natl Acad Sci USA85: 5536–5540PubMedCrossRefGoogle Scholar
  26. Zeitlin L, Olmsted SS, Moench TR, Co MS, Martinell BJ, Paradkar VM, Russell DR, Queen C, Cone RA, Whaley KJ (1998) A humanized monoclonal antibody produced in transgenic plants for immunoprotection of the vagina against genital herpes. Nature Biotechnol16: 1361–1364CrossRefGoogle Scholar

Copyright information

© The Botanical Society of Korea 2001

Authors and Affiliations

  1. 1.Department of Genetic EngineeringChosun UniversityKwang-JuKorea

Personalised recommendations