Journal of Plant Biology

, Volume 42, Issue 4, pp 321–324 | Cite as

Studies on genes and biochemical events induced by carbon-source starvation in plant cells

Preliminary Report


Carrot (Daucus carota L.) suspension cells exhibit a number of physiological responses when carbon sources in the medium are depleted (i.e., carbon-source starvation). We previously reported that activities of several phospholipid catabolic enzymes, such as phospholipase D (PLD) and lipolytic acyl hydrolase (LAH), are induced to provide cells with alternative carbon sources. In this study we report sequence of PLD cDNA. When starvation was prolonged over approximately five days, cells started to die. To analyze the initiation of cell death, we examined the degradation of DNA and activity of DNA endonuclease. Preliminary results showed that DNA degradation occurred at the onset of cell death. Our findings suggest that carrot cells exhibit two different phases-acclimation response and cell death-during starvation. In working toward a long-term objective of understanding the whole scope of biochemical events during starvation, we have also catalogued the genes induced by starvation.


carbon starvation carrot cells phospholipase D 


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Copyright information

© The Botanical Society of Korea 1999

Authors and Affiliations

  • Sehee Kim
    • 1
  • Sunmin Lee
    • 1
  • Taekkyun Lee
    • 1
  • Woosung Lee
    • 1
  1. 1.Department of Biological Science and the Basic Research Science Research InstituteSungkyunkwan UniversitySuwonKorea

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