Journal of Plant Biology

, Volume 40, Issue 2, pp 110–114 | Cite as

Transcription of human lnterleukin-2 gene inNicotiana tabacum driven by CaMV35S promoter

  • Soo Min Park
  • Choo Bong Hong


Human interleukin-2 (IL-2) gene was inserted next of the 35S-transcript promoter of cauliflower mosaic virus in a binary vector and introduced into tobacco genome byAgrobacterium- mediated transformation. Genomic DNA blot analysis of thein vitro regenerated transgenic tobacco confirmed that the introduced human IL-2 gene was successfully integrated into tobacco genome. RNA blot analyses were carried out for the IL-2 transcript to examine expression of the introduced IL-2 gene. Specific signals only from the transgenic tobacco plants indicated that the introduced human IL-2 gene was properly transcribed. The transgenic tobacco plants were self-pollinated and the seeds were harvested. Differences in the level of the transcript were more apparent during the germination of the seeds and between the T2 generation tobacco plants.

Key words

CaMV35S promoter human IL-2 transcript Nicotiana tabacum 


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Copyright information

© The Botanical Society of Korea 1997

Authors and Affiliations

  • Soo Min Park
    • 1
  • Choo Bong Hong
    • 1
  1. 1.Institute for Molecular Biology and GeneticsSeoul National UniversitySeoulKorea

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