Journal of Plant Biology

, Volume 47, Issue 1, pp 27–32 | Cite as

Possible involvement of Ca2+-dependent protein kinases in spore germination of the fernOsmunda Japonica

  • Hiroyuki Kamachi
  • Munenori Noguchi
  • Hiroshi Inoue


Fern gametophyte is a good model system to investigate signal transduction in plant cells. In this work, we examined whether CDPKs are involved in the mechanisms of spore germination of the fernOsmunda japonica. A protein extract from the spores included four CDPK isoforms with relative molecular weights of 56, 53, 49, and 47 kDa, as detected by immunoblot analysis, and they showed CDPK-like activities, as detected by in-gel protein-kinase assay. It was also found that the inhibitors effective on CDPKs, such as a general protein kinase inhibitor, K252a, and a calmodulin antagonist, W-7, largely suppressed the spore germination, and that many proteins of the spores were phosphorylated in vivo in a calcium dependent manner in the period when the spores require external Ca2+ for the germination. Furthermore, we showed that Sr2+ and Mn2+, which could substitute for Ca2+ in the spore germination, were also able to activate theOsmunda CDPKs. From these results, we concluded that CDPKs would participate in the spore germination ofO. japonica.


CDPK fern gametophyte Osmunda japonica spore germination 



Ca2+-dependent protein kinase


ethyleneglycol bis(2-aminoethyl ether)-tetraacetic acid


2-morpholinoethanesulfonic acid


sodium dodecyl sulfate


polyacrylamide gel electrophoresis


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Copyright information

© The Botanical Society of Korea 2004

Authors and Affiliations

  • Hiroyuki Kamachi
    • 1
  • Munenori Noguchi
    • 1
  • Hiroshi Inoue
    • 1
  1. 1.Department of Environmental Biology and ChemistryFaculty of cience, Toyama UniversityToyamaJapan

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