Summary
It has been reported that isolated rat heart myocytes and cardiac mesenchymal cells convert arachidonic acid mainly into three types of prostaglandins (PGs): PGE2, PGF2α, and PGI2 [1]. In addition, we have demonstrated that fresh atrial slices of patients with heart-valve disease contain appreciable quantities of PGE2 and PGF2α [2]. However, there have been no reports on the subcellular localization of the prostaglandin system in heart muscle tissue. The present study was performed to define the distribution of PGE2 in mitochondrial, microsomal, and cytosolic fractions, isolated by differential centrifugation from homogenates of fresh normal rat atrium and ventricle slices. In addition, we determined whether differences exist in PGE2 levels between atrial subcellular fractions and those of ventricular fractions. The results showed that PGE2 was located mainly in the highspeed cytosolic supernatant fraction of the heart homogenates analyzed. Furthermore, PGE2 concentrations (ng/mg protein) were significantly higher in fractions obtained from atrial than those of ventricular tissue.
Similar content being viewed by others
References
Ahumada GG, Sobel BE, Needleman P. Synthesis of prostaglandins by cultured rat heart myocytes and cardiac mesenchymal cells.J Mol Cell Cardiol 1980;12:685–700.
Escobar E, Zamorano B, Gazmuri R. Demonstration of prostaglandin E2 and F2α in atrial tissue of patients with heart disease.Am J Cardiol 1983;52:425.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent.J Biol Chem 1951;193:265–275.
Dray F, Charbonnel B, Maclouf J. Radioimmunoassay of prostaglandins Fα, E1 and E2 in human plasma.Eur J Clin Invest 1975;5:311–318.
Zamorano B. Determinatión de PGE2 por radioinmunoanálisis (RIA) comparación entre el uso de [3H]-PGE2 y [125I]-PGE2. Abstract 2a Jornadas Nationales de la Sociedad Chilena de Biologia y Medicina Nuclear, 1986:1.
Piper P, Vane JR. The release of prostaglandins from lung and other tissue.Ann NY Acad Sci 1971;180:363–385.
Kataoka K, Ramwell PW, Jessup S. Prostaglandins: Localization in subcellular particles of rat cerebral cortex.Science 1967;157:1187–1189.
Mentz P, Pawelski KE, Giessler C, Mest HJ, Mannes F, Rotzoll S. Myocardial biosynthesis of prostacyclin and the influence of cardiac loading and drugs.Biomed Biochim Acta (Germany) 1988;47:244–247.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zamorano, B. Subcellular localization of prostaglandin-E2 in rat heart tissue. Cardiovasc Drug Ther 5, 655–657 (1991). https://doi.org/10.1007/BF03029735
Issue Date:
DOI: https://doi.org/10.1007/BF03029735