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Ricerca in clinica e in laboratorio

, Volume 15, Issue 1, pp 63–69 | Cite as

High-performance liquid chromatographic assay of serotonin in human plasma

  • Bianca Marashini
  • Maria Luisa Biondi
  • Piergiorgio Pietta
  • Angelo Agostoni
Original Contributions
  • 177 Downloads

Summary

A sensitive and reproducible reversed-phase high-performance liquid chromatography with electrochemical detection for determination of serotonin in human plasma is described. The method has an average coefficient of variation of 3.5%; the recovery of serotonin and 5-hydroxy-N-methyltryptamine (internal standard) accounts for 95±2%. Average serotonin concentrations in platelet-free plasma and in platelet-rich plasma from 21 normal subjects (22-63-year-old) ranged from 0.6 to 4.9 ng/ml (mean 2.8±1.3 SD) and from 38.3 to 106.5 ng/108 platelets (mean 66.8±18.9 SD), respectively. The release of endogenous serotonin from human aggregating platelets challenged by collagen or adenosine diphosphate has been quantitated.

Key-words

Biogenic amines High-performance liquid chromatography Serotonin 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Bianca Marashini
    • 1
  • Maria Luisa Biondi
    • 1
  • Piergiorgio Pietta
    • 2
  • Angelo Agostoni
    • 1
  1. 1.Istituto di Scienze BiomedicheClinica Medica V Ospedale S. PaoloMilanoItaly
  2. 2.Dipartimento di Scienze e Tecnologie Biomediche, Sezione di Chimica OrganicaUniversità degli Studi di MilanoItaly

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