Crystal Structure of Hydrogenated Canola Oil
Scanning electron microscopy was used to study the crystal structure of hydrogenated Canola oil. Fixation of the samples was carried out at 10 C using osmium tetroxide vapor in a sealed container. Tristearin was used as a standard and was prepared in its three reported polymorphic forms α, β′ and β. Crystallization from ethyl acetate gave either a mixture of β′ and β or pure β′ polymorphic forms depending on the concentration of tristearin. Micrographs of these forms were obtained showing the morphology of the crystals. A Canola sample which was selectively hydrogenated to an iodine value of 70 was detergent fractionated, washed, dried and subjected to the same fixation procedure. Micrographs were obtained of its predominant β-polymorphic form.
KeywordsOsmium Tetroxide Polymorphic Form Tristearin Polarize Light Microscope Dioctylsulfosuccinate
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