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Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor

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Abstract

In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method and utilized repeatedly by regeneration. The monoclonal antibody, ‘A18’, was selected and employed for the high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 103 cells/ml or 50 cells were detected by the SPR biosensor.

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Authors and Affiliations

  1. BioNanotechnology Research Center, KRIBB, 305-806, Daejeon, Korea

    Hyou-Arm Joung, Junhyoung Ahn, Bong Hyun Chung & Min-Gon Kim

  2. Department of Chemical Engineering, Chungnam National University, 305-701, Daejeon, Korea

    Hyou-Arm Joung & Ho-Suk Choi

  3. Department of Food Science and Technology, Gyeongsang National University, 660-701, Jinju, Korea

    Won-Bo Shim & Duck-Hwa Chung

  4. Department of Food Science and Technology, Chung-Ang University, 155-756, Seoul, Korea

    Sang-Do Ha & Keun-Sung Kim

  5. Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies, 449-791, Yongin, Korea

    Kyu-Ho Lee

  6. Department of Biological Sciences, College of Natural Science, Sungkyunkwan University, 440-746, Suwon, Korea

    Cheol-Ho Kim

  7. Department of Food Science and Technology, Chungbuk National University, 361-763, Cheongju, Korea

    Kwang-Yup Kim

Authors
  1. Hyou-Arm Joung
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  2. Won-Bo Shim
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  3. Duck-Hwa Chung
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  4. Junhyoung Ahn
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  7. Sang-Do Ha
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  8. Keun-Sung Kim
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  9. Kyu-Ho Lee
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  11. Kwang-Yup Kim
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  12. Min-Gon Kim
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Correspondence to Min-Gon Kim.

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Joung, HA., Shim, WB., Chung, DH. et al. Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor. Biotechnol. Bioprocess Eng. 12, 80–85 (2007). https://doi.org/10.1007/BF03028630

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  • DOI: https://doi.org/10.1007/BF03028630

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