Biotechnology and Bioprocess Engineering

, Volume 11, Issue 4, pp 282–287 | Cite as

Purification and characterization of a recombinantCaulobacter crescentus epoxide hydrolase

  • Seungha Hwang
  • Hyejin Hyun
  • Byoungju Lee
  • Youngseub Park
  • Eun Yeol Lee
  • Chayong Choi


ACaulobacter crescentus epoxide hydrolase (CCEH) from a recombinantEscherichia coli was purified to homogeneity using a three-step procedure. The CCEH protein was purified 7.3-fold with a 22.9% yield in overall activity. The optimal reaction temperature and pH were determined to be 37°C and pH 8.0, respectively. The addition of 10% (v/v) dimethylsulfoxide as a cosolvent improved the enantioselectivity of CCEH for a batch kinetic resolution of racemic indene oxide.


chiral styrene oxide chiral indene oxide Caulobacter crescentus epoxid hydrolase enzyme purification 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2006

Authors and Affiliations

  • Seungha Hwang
    • 1
  • Hyejin Hyun
    • 2
  • Byoungju Lee
    • 2
  • Youngseub Park
    • 1
  • Eun Yeol Lee
    • 3
  • Chayong Choi
    • 2
  1. 1.Department of Chemical EngineeringPohang University of Science and TechnologyPohangKorea
  2. 2.Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of EngineeringSeoul National UniversitySeoulKorea
  3. 3.Department of Food Science and TechnologyKyungsung UniversityBusan

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