Journal of the American Oil Chemists’ Society

, Volume 66, Issue 9, pp 1386–1389 | Cite as

An in vitro method for evaluation of the irritancy of antonio surfactants

  • A. S. Anavkar
  • C. V. Natraj
Technical Surfactants & Detergents Technical


Extraction of Coomassie Brilliant Blue R250 dye from gelatin by anionic surfactants was found to be proportional to the concentration, duration of extraction, and the nature of the surfactant. Under a given set of conditions, the decreasing order of dye extraction was SLS > LAS > sodium laurate > AOS ≅ SLES. This order agrees well with the decreasing order of irritancy of these surfactants. For a homologous series such as sodium salts of carboxylic acids, there was a sharp maximum in dye extraction for laurate. This coincides with the reported order of irritancy for soaps of fatty acids. Substitution of gelatin by agar or Coomassie Brilliant Blue by eosin abolished this specificity. Thus, the measurement of extraction of Coomassie Brilliant Blue R250 from gelatin is a reproducible and simple method for estimating the irritancy of anionic surfactants.


Surfactant Gelatin Stratum Corneum Brilliant Blue Anionic Surfactant 
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Copyright information

© American Oil Chemists’ Society 1989

Authors and Affiliations

  • A. S. Anavkar
    • 1
  • C. V. Natraj
    • 1
  1. 1.Hindustan Lever Research CentreBombayIndia

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