Japanese Sardine Oil as a Source of 16:3(n-4) and 16:4(n-l) Fatty Acids
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Open tubular gas liquid Chromatographic (GLC) analysis of fatty acids in Japanese sardine oil showed the contents of 1.57% hexadecatrienoic acid (16:3) and 2.55% hexadecatetraenoic acid (16:4). Concentrations of 16:3 and 16:4 were carried out through the urea adduct method, argentation thin layer chromatography (TLC) and reversed phase TLC, with the purities of 16:3 and 16:4 samples obtained being both higher than 92%. They were recognized as 16:3(n-4) and 16:4(n-1) by nuclear magnetic resonance analyses of the methyl esters and GLC analyses of the partially hydrazine hydrogenated products and their oxidative ozonolysis products. The structures were supported by the results of gas chromatographic-mass spectrometric analyses of the pyrrolidides. Other isomers of 16:3 and 16:4 have not been detected in the GLC analysis of the methyl esters. These results indicate that sardine oil is useful as a source of these fatty acids.
KeywordsOlefinic Bond Polyenoic Acid Japanese Sardine Reversed Phase Thin Layer Chromatography Methyl Decanoate
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