Reproductive Medicine and Biology

, Volume 5, Issue 2, pp 145–153 | Cite as

Effect of L-ascorbic add supplementation on testicular oxidative stress and endocrine disorders in mature male rats exposed to intensive swimming exercise

  • Prabhat Kumar Samanta
  • Indranil Manna
  • Kuladip Jana
Original Article Andrology


In order to investigate the ameliorative potential of L-ascorbic acid on intensive swimming exercise induced testicular oxidative stress, 18 Wistar male rats (age: 3 months, weight: 127.5 ± 5.3 g) were randomly divided into the following groups: (i) control group (CG,n = 6); (ii) experimental group (EG,n = 6); and (iii) supplemented group (SG,n = 6). An exercise protocol of 3 h swimming per day, five days per week was followed for 6 weeks in EG and SG with no exercise in CG. In SG, L-ascorbic acid was supplied orally at a dose of 25-mg/kg of bodyweight each day for 6 weeks. A significant decrease (P < 0.05) was noted in paired testicular weights, epididymal sperm count, testicular Δ5, 3β-hydroxyseroid dehydrogenase, 17β-hydroxyseroid dehydrogenase, plasma levels of testosterone luteinizing hormone, follicle stimulating hormone, prolactin, the numbers of preleptotine spermatocytes, midpachytene spermatocytes and stage 7 spermatids of stage VII seminiferous epithelium cycle in EG when compared with CG. A significant elevation (P < 0.05) in plasma corticosterone and testicular content of malondialdehyde along with a significant reduction (P < 0.05) in glutathione, ascorbic acid, α-tocopherol, superoxide dismutase, catalase and glutathione-peroxidase, and glutathione-S-transferase were noted in testes of EG compared with CG. No significant change was noted in final bodyweight or numbers of spermatogonia-A among the groups. Furthermore, L-ascorbic acid supplementation restored the above parameters to the control level.


It can be concluded that intensive swimming exercise induced oxidative stress causes dysfunctions in the male reproductive system, which can be protected by L-ascorbic acid.

Key words

antioxidants L-ascorbic acid oxidative stress spermatogenesis steroidogenesis swimming exercise 


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Copyright information

© the Japan Society for Reproductive Medicine 2006

Authors and Affiliations

  • Prabhat Kumar Samanta
    • 1
  • Indranil Manna
    • 1
  • Kuladip Jana
    • 1
    • 2
  1. 1.Department of Surgery & RadiologyWest Bengal University of Animal & Fishery SciencesIndia
  2. 2.Institute of Molecular MedicineBengal Intelligent Park LtdCalcuttaIndia

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