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Journal of Solid-Phase Biochemistry

, Volume 1, Issue 1, pp 51–65 | Cite as

Evidence for the induction of a conformational change of trypsin by a specific substrate at pH 8.0

  • Rosa Uy
  • Victor S. H. Liu
  • Garfield P. Royer
Article
  • 29 Downloads

Abstract

A method is described that permits the covalent attachment of enzymes to solid matrices in the presence of saturating levels of a specific substrate under conditions where the enzyme is optimally active. Bovine trypsin immobilized on glass and agarose in the presence of saturating levels of N-α-benzoyl-L-arginine ethyl ester differs in a number of respects from the enzyme bound in the absence of substrate. Trypsin bound in the presence of substrate (ES form) is attached to porous glass through three more side chains than the enzyme coupled in the absence of substrate (E form). The rate of inactivation by iodoacetamide for the ES form is much greater than that for the E form. The two enzyme forms differ also in their reactivity with TLCK and DFP, active site-directed inhibitors. The pH dependences of the ES and the E forms bound to agarose differ; the pH optima are 9.0 and 8.5 for the ES and E forms, respectively. The thermal denaturation of the agarose-trypsins occurs in two distinct steps. The inactivation rate of the ES form is significantly slower than the E form in both stages. Also, for the hydrolysis of both N-α-benzoyl-L-arginine ethyl ester and N-α-benzoyl-Larginine amide, the Km values for the ES form were lower than the Km values for the E form. Our data suggest that a substrate-induced conformatonal change occurs with trypsin, and that this conformation is stabilized when the enzyme is bound by multiple attachments to a solid support. These findings support the “induced-fit” theory in general and lend support to the interpretation of the activation of trypsin by methyl guanidine that is part of a specific substrate (Inagami, T., and Hatano, H. [1969] J. Biol. Chem. 244, 1176).

Keywords

Trypsin Iodoacetamide Porous Glass Enzyme Form Bovine Trypsin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1976

Authors and Affiliations

  • Rosa Uy
    • 1
  • Victor S. H. Liu
    • 1
  • Garfield P. Royer
    • 1
  1. 1.Department of BiochemistryThe Ohio State UniversityColumbus

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