Skip to main content
SpringerLink
Log in

Affinity purification methods IV. The amino(2-hydroxy)propyl ether-leash in bioselective sorbents

  • Published:
Journal of Solid-Phase Biochemistry Aims and scope Submit manuscript

Abstract

The amino(2-hydroxy)propyl ether derivative of agarose is obtained by treating the gel in 0.3 M NaOH with epibromohydrin at 30°C for 4 h, followed by a 16-h reaction with 1 M NH4OH. The derivative is nonionic and chemically stable. Established coupling methods can be used to attach biochemical substances to this amino-leash. A variety of reaction parameters have been studied to optimize the epibromohydrin reaction step. It is shown that the amino-leash agarose gel can be readily used to prepare hydrophobic sorbents, affinity sorbents, immobilized enzymes, and immunosorbents.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wilchek, M., andMiron, T. (1974) Mol. Cell. Biochem. 4:181–187.

    Article  CAS  Google Scholar 

  2. O’Carra, P., Barry, S., andGriffin, T. (1974) FEBS Lett. 43: 169–175.

    Article  CAS  Google Scholar 

  3. Jost, R., Miron, T., andWilchek, M. (1974) Biochim. Biophys. Acta 362: 75–82.

    CAS  Google Scholar 

  4. Nishikawa, A. H., andBailon, P. (1975) Arch. Biochem. Biophys. 168: 576–584.

    Article  CAS  Google Scholar 

  5. Wilchek, M. (1975) Fed. Proc. Fed. Am. Soc. Exp. Biol. 34: 546 (abstract).

    Google Scholar 

  6. Tesser, G. I., Fisch, H-U., andSchwyzer, R. (1974) Helv. Chim. Acta 57: 1718–1730.

    Article  CAS  Google Scholar 

  7. Ghetie, V., andSchell, H. D. (1967) Rev. Roum. Biochim. 4: 179–184.

    CAS  Google Scholar 

  8. Schell, H. D., andGhetie, V. (1968) Stud. Cercet. Biochim. 11: 69–74.

    CAS  Google Scholar 

  9. Porath, J., Janson, J-C, andLaas, T. (1971) J. Chromatogr. 60: 167–177.

    Article  CAS  Google Scholar 

  10. Sundberg, L., andPorath, J. (1974) J. Chromatogr. 90: 87–98.

    Article  CAS  Google Scholar 

  11. Fairbourne, A.,Gibson, G. P., andStephens, D. W. (1932) J. Chem. Soc. (London), 1965–1972.

  12. Hixson, H. F., andNishikawa, A. H. (1973) Arch. Biochim. Biophys. 154: 501–509.

    Article  CAS  Google Scholar 

  13. Inman, J. K., andDintzis, H.M. (1969) Biochemistry 8: 4074–4082.

    Article  CAS  Google Scholar 

  14. Nishikawa, A. H., andBailon, P. (1975) Anal. Biochem. 64: 268–275.

    Article  CAS  Google Scholar 

  15. Anderson, G. W., Zimmerman, J. E., andCallahan, F. M. (1967) J. Am. Chem. Soc. 89: 5012–5017.

    Article  CAS  Google Scholar 

  16. Worthington Enzyme Manual (1972) Worthington Biochem. Corp., Freehold, New Jersey, p. 129.

  17. Ross, W. C. J. (1950) J. Chem. Soc. (London), 2257–2272.

  18. Hjerten, S., Rosengren, J., andPahlman, S. (1974) J. Chromatogr. 101: 281–288.

    Article  CAS  Google Scholar 

  19. Nishikawa, A. H.,Bailon, P., andRamel, A. H. J. Macromol. Sci. (in press).

  20. Nishikawa, A. H., andBailon, P. (1975) Anal. Biochem. 68: 274–280.

    Article  CAS  Google Scholar 

  21. Campbell, D. H.,Garvey, J. S.,Cremer, N. E., andSussdorf, D. H. (1970) Methods in Immunology, 2nd ed., W. A. Benjamin, New pp. p. 189–191.

  22. Guilford, H. (1973) Chem. Soc. Rev. 2: 249–270.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemical Research Department, Hoffmann-La Roche, Inc., 07110, Nutley, New Jersey

    A. Hirotoshi Nishikawa & Pascal Bailon

Authors
  1. A. Hirotoshi Nishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pascal Bailon
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nishikawa, A.H., Bailon, P. Affinity purification methods IV. The amino(2-hydroxy)propyl ether-leash in bioselective sorbents. Journal of Solid-Phase Biochemistry 1, 33–49 (1976). https://doi.org/10.1007/BF03006135

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03006135

Keywords

Search

Navigation