Rendiconti Lincei

, Volume 3, Issue 3, pp 247–255 | Cite as

Chromosome banding by restriction enzyme digestion distinguishes between Mus domesticusand Mus musculuskaryotypes

  • Silvia Garagna
  • Carlo Alberto Redi
  • Paola Veneroni
  • Ernesto Capanna
  • E. Capanna


The results obtained after restriction enzymes digestion ofMus domesticus andMus musculus chromosomes, showed regional chromosomal staining differences suitable for distinguishing between trypsin Giemsa homosequential banding patterns. These differences are particularly clear at the pericentromeric level where the satellite DNA is located. These data support the idea that major differences exist in the DNA composition and organization of the satellite DNA betweendomesticus andmusculus genomes.

Key words

Satellite DNA Restriction enzyme banding Mus. 

Il bandeggio cromosomico ottenuto con enzimi di restrizione rivela differenze tra cariotipi di Mus domesticuse di Mus musculus


L’analisi dei risultati ottenuti, utilizzandoin situ enzimi di restrizione in grado di riconoscere e tagliare specifiche sequenze nucleotidiche, ha permesso di mettere in evidenza differenze in grado di distinguere i cariotipi diMus domesticus e diMus musculus, cariotipi che appaiono omosequenziali utilizzando le convenzionali tecniche di G-bandeggiamento. L’analisi dei patterns di digestione enzimatica a livello delle porzioni pericentromeriche dei cromosomi didomesticus e dimusculus ha inoltre confermato la differenza strutturale esistente tra i DNA satellite delle due specie.


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Copyright information

© Accademia nazionale dei Lincei 1992

Authors and Affiliations

  • Silvia Garagna
    • 2
  • Carlo Alberto Redi
    • 2
  • Paola Veneroni
    • 2
  • Ernesto Capanna
  • E. Capanna
    • 1
  1. 1.Dipartimento di Biologia Animale e dell’UomoUniversità degli Studi di Roma «La SapienzaRoma
  2. 2.Dipartimento di Biologia Animale e Centro di Studio per l’lstochimica del C.N.RUniversità degli Studi di PaviaPavia

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