Skip to main content
SpringerLink
Log in

The Avidin-Biotin complex in solid phase radioimmunoassays

  • Published:
Journal of Solid-Phase Biochemistry Aims and scope Submit manuscript

Abstract

A solid-phase radioimmunoassay is described in which biotin conjugated antibodies are used to replace125I labeled antibodies to individual antigens. The amount of antigen present is subsequently determined by the binding of125I-labeled avidin. This method is appealing for a variety of reasons. (a) Only one125I-labeled protein (avidin) need be prepared and characterized for all affinity systems, (b) There is no need to purify individual antibodies. (c) Biotin can be attached to antibodies under mild conditions. (d) The size, physical characteristics, and biological activity of the biotin-derived antibody are only nominally affected. (e) The biotin-avidin complex is of exceptionally high affinity and stability, (f) Introduction of biotin groups into the antibodies leads to amplified radioactive tracer binding, (g) Avidin and biotin are commercially available.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bayer, E. A., andWilchek, M. (1978) Trends Biochem. Sci. 3: N257–259.

    Google Scholar 

  2. Bayer, E. A., andWilchek, M. (1980) Method Biochem. Anal. 26: 1–45.

    Article  CAS  Google Scholar 

  3. Hiezmann, H., andRichards, F. M. (1974) Proc. Natl. Acad. Sci. USA 71: 3537–3541.

    Article  Google Scholar 

  4. Bayer, E. A., Skutelsky, E., Wynne, D., andWilchek, M. (1976) J. Histochem. Cytochem. 24: 933–939.

    CAS  Google Scholar 

  5. Bayer, E. A., Wilchek, M., andSkutelsky, E. (1976) FEBS Lett. 68: 240–244.

    Article  CAS  Google Scholar 

  6. Angerer, L., Davidson, N., Murphy, W., Lynch, D., andAttardi, G. (1976) Cell 9: 81–90

    Article  CAS  Google Scholar 

  7. Becker, J. M., andWilchek, M. (1972) Biochim. Biophys. Acta. 264: 165–170.

    CAS  Google Scholar 

  8. Wilchek, M., Bocchini, V., Becker, M., andGivol, D. (1971) Biochemistry 10:2828–2834.

    Article  CAS  Google Scholar 

  9. Wynne, D., Wilchek, M., andNovogrossky, A. (1976) Biochem. Biophys. Res. Commun. 68: 730–739.

    Article  CAS  Google Scholar 

  10. Guesdon, J. L., Ternynck, T., andAvrameas, S. (1979) J. Histochem. Cytochem. 27:1131–1139.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics The Weizmann Institute of Science Rehovot, Israel

    Meir Wilchek

Authors
  1. Meir Wilchek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wilchek, M. The Avidin-Biotin complex in solid phase radioimmunoassays. Journal of Solid-Phase Biochemistry 5, 193–195 (1980). https://doi.org/10.1007/BF03000655

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03000655

Keywords

Search

Navigation