Cumulative Bone Marrow Tromal damage caused by X-Irradiation and Cytosine-Arabinoside in Leukemic mice

  • Zina Ben-ishay
  • Gregor Prindull
  • Sara Yankelev
  • Sara Sharon


A study of treated murine acute myeioid leukemia (AML) with an emphasis on the bone marrow stromal function is reported. Leukemia was induced in C57BI mice through intraperitoneal (i.p.) inoculation of C-1498 myelogenous leukemic cells. The leukemic mice were administered: (1) total body lethal X-irradiation (t.b.i); (2) two i.p. cytosine-arabinoside (Ara-C) injections followed by X-irradiation. Control mice received similar regimens. Bone marrow of experimental and control mice was processed for stromal cell cultures (SCC) andin vitro engraftment of hematopoietic cells onto the cultures. The results of this study indicate that the bone marrow stromal deficiency which occurs in leukemia is aggravated by Ara-C and irradiation treatments. Moreover, SCC of treated leukemic mice sustainin vitro hematopoiesis only to a limited degree. Stromal deficiency, as possible cause for graft failure in bone marrow transplanted leukemic patients, is discussed.

Key words

Bone-marrow stroma Murine acute myeloid leukemia Cytosine-arabinoside X-irradiation 


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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Zina Ben-ishay
    • 1
  • Gregor Prindull
    • 2
  • Sara Yankelev
    • 3
  • Sara Sharon
    • 1
  1. 1.Laboratory of Experimental Hematology and Department of Anatomy & EmbryologyHebrew University-Hadassah Medical SchoolJerusalemIsrael
  2. 2.Department of PediatricsUniversity of GöttingenGöttingenFederal Republic of Germany
  3. 3.Genetics Institute Inc.Cambridge.USA

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