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Enzymic analysis of organophosphate insecticides using an enzyme thermistor

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Abstract

Quantitative analysis of water soluble insecticides using an enzyme thermistor was examined for organophosphates. A procedure based on an insecticide hydrolyzing enzyme was found suitable for continuous determination of insecticides at concentrations down to 30 µM. A more sensitive procedure, < 3.4µM, suitable for discontinuous monitoring was developed using reversible immobilization of acetylcholine esterase.

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Authors and Affiliations

  1. Bio-P, A4/347, Postfach 4119, D-6100, Darmstadt 2, West Germany

    Erwin Rieke

  2. Department of Botany and Microbiology, University of Oklahoma, 73019, Norman, Oklahoma

    Doug Munnecke

  3. Biochemistry 2, Chemical Center University of Lund, P.O. Box 740 5-220 07, Lund 7, Sweden

    Bo Mattiasson & Klaus Mosbach

Authors
  1. Bo Mattiasson
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  2. Erwin Rieke
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  3. Doug Munnecke
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  4. Klaus Mosbach
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Mattiasson, B., Rieke, E., Munnecke, D. et al. Enzymic analysis of organophosphate insecticides using an enzyme thermistor. Journal of Solid-Phase Biochemistry 4, 263–270 (1979). https://doi.org/10.1007/BF02998680

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  • DOI: https://doi.org/10.1007/BF02998680

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