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Journal of Solid-Phase Biochemistry

, Volume 4, Issue 4, pp 253–262 | Cite as

Direct coupling of glucose oxidase to platinum and possible use forin vivo glucose determination

  • L. B. Wingard
  • D. Ellis
  • S. J. YAO
  • J. G. Schiller
  • C. C. Liu
  • S. K. Wolfson
  • A. L. Drash
Article

Abstract

Glucose oxidase was attached to platinum-platinum oxide screens via alkylamine silaneglutaraldehyde coupling. The amount of immobilized enzyme was equivalent to 0.0031 µg of soluble glucose oxidase per cm2 of screen surface. The platinum-silane-glutaraldehydeenzyme screens were tested potentiometrically in buffered glucose solutions, with respect to a Ag/AgCl reference electrode. The results were expressed as the difference in potential for the enzyme screens placed in buffer containing glucose and placed in plain buffer. This difference in potential was related linearily to the logarithm of the glucose concentration over the range 5–150 mg glucose/100 ml. The source of the potential may be due to the decomposition of hydrogen peroxide produced by the glucose oxidase catalyzed oxidation of glucose. The approach is being studied for possible development of an implantable sensor for continuousin vivo monitoring of glucose levels.

Keywords

Immobilize Enzyme Glucose Oxidase Direct Coupling Platinum Oxide Potentiometric Response 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Galloway, J. A. (1976) Diabetes Mellitus, 7th edn., Eli Lilly and Co., Indianapolis, pp. 1–10.Google Scholar
  2. 2.
    Caraway, W. T. (1976)In Fundamentals of Clinical Chemistry,TIETZ, N. W. (ed.), W. B. Saunders, Philadelphia, pp. 242–250.Google Scholar
  3. 3.
    Fogt, E. J., Dodd, L. M., Jenning, E. M., andClemens, A. H. (1978) Clin. Chem. 24: 1366.Google Scholar
  4. 4.
    Gough, D. A, Aisenberg, S., Giner, J., andSoeldner, J. S. (1977)In Blood Glucose Monitoring,Kruse-Jarres, J. D., andMolnar, G. D. (eds.), Georg Thieme Publishers, Stuttgart, pp. 10–22.Google Scholar
  5. 5.
    Reitnauer, P. G. (1975) Z. Med. Labortechnik, 16: 284.Google Scholar
  6. 6.
    Layne, E. C. Schultz, R. D., Thomas L. J. JR., Slama, G., Sayler, D. F., andBessman, S. P. (1976) Diabetes 25: 81.CrossRefGoogle Scholar
  7. 7.
    Liu, C. C. Wingard L. B., JR., Wolfson, S.K., JR., Yao, S. J., Drash, A. L., andSchiller, J. G. (1979) Bioelectrochem. Bioenergetics 6:21.CrossRefGoogle Scholar
  8. 8.
    Gillett, R. andGull, K. (1972) Histochemie 30: 162.CrossRefGoogle Scholar
  9. 9.
    Lenhard, J. R., andMurray, R. W. (1977) J. Electroanal. Chem. 78: 195.CrossRefGoogle Scholar
  10. 10.
    Weetall, H.H. andFilbert, A. M. (1974)In Methods in Enzymology, Vol. 34,Jakoby, W. B., andWIlchek, M. (eds.), Academic, New York, pp. 59–72.Google Scholar
  11. 11.
    Huggett, A. S. G., andNixon, D. A. (1957) Lancet 2: 368.CrossRefGoogle Scholar
  12. 12.
    Untereker, D. F., Lennox, J. C. WIER, L. M., MOSES, P. R., andMurray, R. W. (1977) J. Electroanal. Chem. 81: 309.CrossRefGoogle Scholar
  13. 13.
    Weetall, H. H., andHersh, L. S. (1970) Biochim. Biophys. Acta 206: 54.Google Scholar
  14. 14.
    Anson, F. C. (1961) Anal. Chem. 33: 934.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 1979

Authors and Affiliations

  • L. B. Wingard
    • 1
  • D. Ellis
    • 1
  • S. J. YAO
    • 2
  • J. G. Schiller
    • 4
  • C. C. Liu
    • 4
  • S. K. Wolfson
    • 2
  • A. L. Drash
    • 3
  1. 1.Departments of PharmacologySchool of Engineering University of PittsburghPittsburgh
  2. 2.NeurologicalDepartments of Pharmacology,School of Engineering University of PittsburghPittsburgh
  3. 3.PediatricsDepartments of Pharmacology,School of Engineering University of PittsburghPittsburgh
  4. 4.School of Medicine and Department of Chemical and Petroleum EngineeringDepartments of Pharmacology,School of Engineering University of PittsburghPittsburgh

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