Some possibilities for the immobilization of enzymes in poly(vinyl acetate-co-ethylene) tubes
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The inside surface of poly(vinyl acetate-co-ethylene) tubes was coated with poly(vinyl alcohol) in the absence as well as in the presence of terephthalaldehyde as crosslinking agent. A hydrophilic inside tube surface was obtained, which was modified by reaction with 2, 4, 6-trichloro-s-triazine resp. with 2-(3-aminophenyl)-l,3-dioxolane. The latter method gave reactive tube supports after diazotization resp. after activation with glutardialdehyde. Furthermore, the amino derivative could be reacted with enzyme aggregates formed by reaction of glucose oxidase with 4-(2,3-epoxypropoxy)-benzaldehyde. Trypsin and glucose oxidase were immobilized onto the activated inside tube surfaces, and the properties of the immobilized enzymes were studied. The flow patterns of tube-immobilized trypsin were studied. The application of tube-immobilized glucose oxidase for automated glucose analysis was tested.
KeywordsImmobilize Enzyme Glucose Oxidase Immobilize Trypsin Immobilize Glucose Oxidase Glutardialdehyde
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