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Chinese Journal of Cancer Research

, Volume 2, Issue 4, pp 47–51 | Cite as

Generation and characterization of two monoclonal antibodies against cytokeratins

  • Zhao Jingfeng 
  • Shao Wenzhao 
  • Li Xibin 
  • Liu Zhengmei 
  • Sun Meizhen 
  • Zhang Ying 
  • Chen Lin 
Basic Investigations
  • 12 Downloads

Abstract

Thirty-five hybridoma cell lines against cytokeratins, isolated from Hela cells and human cellus respectively, were generated by fusion of immunized spleen cells of BALB/C mice with P3×63-Ag8.653, a mouse myeloma cell line. Two of them (HI and C53) were characterized by indirect immunofluorescence, ABC inununostaining and immunoblotting. The results of immunofluorescence and ABC immunostaining suggested that both monoclonal antibodies were specific for keratin-type intermediate filaments. However, the two monoclonal antibodies showed different specificities in normal tissues and neoplasms as observed on both frozen and deparaffinized sections. In normal tissues, H1 stained transitional epithelium and all types of simple epithelium except endothelium and mesothelium but did not stain stratified squamous epithelium. In contrast, C35 recognized only stratified squamous epithelium, but failing to react with simple epithelium. Both monoclonal antibodies did not react with nonepithelia cells and tissues. In neoplasms, HI stained varieties of adenocarcinomas and C35 recognized merely squamous cell carcinomas. Therefore, all the epithelial tissues can nearly be recognized by combination of the two monoclonal antibodies. AH the results indicated that H1 and C35 can be used in cell biology and histology studies, and can be used in differential diagnosis of adenocarcinoma and squamous cell carcinoma in pathology.

Keywords

Renal Cell Carcinoma Hela Cell Squamous Epithelium Glandular Epithelium Stratify Squamous Epithelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chinese Journal of Cancer Research 1990

Authors and Affiliations

  • Zhao Jingfeng 
    • 1
  • Shao Wenzhao 
    • 1
  • Li Xibin 
    • 1
  • Liu Zhengmei 
    • 1
  • Sun Meizhen 
    • 1
  • Zhang Ying 
    • 1
  • Chen Lin 
    • 1
  1. 1.Department of Cell BiologyBeijing Neurosurgical InstituteChina

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