The effects of estradiol on breast cancer cell lines
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The estradiol effects on breast cancer cell lines including estrogen receptors (ER) positive and negative were studied with flow cytometry analysis, scanning and transmission electron microscopy (SEM and TEM), immunogold and immunofluorescence staining techniques. The results showed that estradiol markedly stimulated the division and proliferation of the ER(+) MCF-7 cells at 10 nM, but had no marked effect on the cell cycle of the ER(−) H466B cells at the same concentration, and that tamoxifen inhibited the stimulation of estradiol on MCF-7 cells. Estradiol obviously influenced the ultrastructure of MCF-7 cells. Immunocytochemical localization of epidermal growth factor receptor (ECFR) on the MCF-7 cell membrane surface indicated that one of the mechanisms involving the growth of MCF-7 breast cancer cell and the stimulating effect on MCF-7 cells growth by estradiol is autocrine secretion.