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The effects of estradiol on breast cancer cell lines

  • Zhang Xuemin 
  • Chen Dehui 
  • Lu Yinglin 
  • Zhang Heqiu 
  • Guo Ning 
  • Wang Yihua 
Basic Investigations

Abstract

The estradiol effects on breast cancer cell lines including estrogen receptors (ER) positive and negative were studied with flow cytometry analysis, scanning and transmission electron microscopy (SEM and TEM), immunogold and immunofluorescence staining techniques. The results showed that estradiol markedly stimulated the division and proliferation of the ER(+) MCF-7 cells at 10 nM, but had no marked effect on the cell cycle of the ER(−) H466B cells at the same concentration, and that tamoxifen inhibited the stimulation of estradiol on MCF-7 cells. Estradiol obviously influenced the ultrastructure of MCF-7 cells. Immunocytochemical localization of epidermal growth factor receptor (ECFR) on the MCF-7 cell membrane surface indicated that one of the mechanisms involving the growth of MCF-7 breast cancer cell and the stimulating effect on MCF-7 cells growth by estradiol is autocrine secretion.

Keywords

Epidermal Growth Factor Receptor Estrogen Receptor Tamoxifen Estradiol Breast Cancer Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Patrice V, et al. Effect of estradiol on the ultrastructure of the MCF-7 human breast cancer cells in culture. Cancer Research 1982; 42: 667.Google Scholar
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    Sporn MB, et al. Autocrine secretion and malignant transformation of cells. N Engl J Med 1980; 303:878.PubMedGoogle Scholar
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    Kasid A, et al. Estrogen and oncogene mediated growth regulation of human breast cancer cells. J Steroid Biochem 1987; 27:465PubMedCrossRefGoogle Scholar

Copyright information

© Chinese Journal of Cancer Research 1990

Authors and Affiliations

  • Zhang Xuemin 
    • 1
  • Chen Dehui 
    • 1
  • Lu Yinglin 
    • 1
  • Zhang Heqiu 
    • 1
  • Guo Ning 
    • 1
  • Wang Yihua 
    • 1
  1. 1.Academy of Military Medical SciencesBeijing

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