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Chinese Journal of Cancer Research

, Volume 2, Issue 4, pp 5–7 | Cite as

Transforming activity of DNA from human esophageal cancer and the identification of the transforming gene

  • Liang Keli 
  • Li Zexiao 
  • Lin Zhuotang 
  • Gao Qixin 
  • Jiang Dongxia 
  • Li Jinzhou 
  • Liu Dongliang 
  • Chen Yuanqing 
  • Gu Jianren 
Basic Investigations
  • 17 Downloads

Abstract

Rat-1 cells were transfected with DNA from human esophageal cancer 2K, 4K, 6K, 7K, 8K. The transforming foci were obtained and the transforming cell lines were established. The cell lines can form larger colony in soft agar. Those nude mice injected subcutaneously with the cells suffered from larger fibrous sarcoma. This indicates that the cell lines have carcinogenicity. The experimental results suggest that human DNA sequence and human Ha-ras special 616Kb (BamHI) band are present in the DNA of the transforming cells. The over-expression ofras gene products P21 were found in the tissues of exophageal cancer, the tissues adjacent to tumor and the transforming cells.

Keywords

Esophageal Cancer Nude Mouse Soft Agar Large Colony Molecular Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chinese Journal of Cancer Research 1990

Authors and Affiliations

  • Liang Keli 
    • 1
  • Li Zexiao 
    • 1
  • Lin Zhuotang 
    • 1
  • Gao Qixin 
    • 1
  • Jiang Dongxia 
    • 1
  • Li Jinzhou 
    • 1
  • Liu Dongliang 
    • 1
  • Chen Yuanqing 
    • 1
    • 2
  • Gu Jianren 
    • 1
    • 2
  1. 1.Henan Tumor InstituteZhengzhou
  2. 2.Shanghai Cancer InstituteChina

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