Studies of nucleolar organizer regions and mucin histochemistry in ulcerative colitis
The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to investigate the dysplasia and cancaration in ulcerative colitis including 58 cases of biopsy specimens and three cases operative specimens. The numbers of AgNOR have been gradully increased with the grades of dasplasis. Similarly the percentage of sialomucin having vicinal diols on side chain of sialic acid also gradully increased with the grades of dysplasia. The AgNOR reflects rDNA transcriptional activity responsible for degrees of differentiation of cell. Epithelial cells secreting a heterogeneous mucin, could be taken as a signal of abnormal cellular differentiation. AgNOR and mucin chages might be assumed as a criteria in representing malignant transformation.
KeywordsUlcerative Colitis Nucleolar Organizer Region Severe Dysplasia Normal Colonic Mucosa Mild Dysplasia
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