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Comparative study of phosphotyrosyl protein phosphatase of mice normal liver, regenerating liver, and mice H22a hepatoma ascites cell

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Chinese Journal of Cancer Research

Abstract

In regenerating liver of mice, marked increase of the activity of phosphotyrosyl protein phosphatase (PTPP) in cytosol was observed. The PTPP activity varied with time and reached the highest level between 24 to 48 hours after partial hepatectomy. In H22a cells the PTPP activity found in every subcellular fraction was lower than that of the normal liver. The PTPP activity was mostly concentrated in lysosomes of normal liver, but mainly distributed in nucleus, cytosol and microsome of regenerating liver. In H22a cells PTPP activity seemed distribute evenly. Five similar major PTPP peaks (I–V) were obtained on DEAE cellulose chromatography of cytosols from all three of liver cells studied. However, two additional PTPP peaks, a and b, were also obtained from cytosol of liver.

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Authors and Affiliations

  1. Laboratory of Molecular Biology and Biochemistry, Beijing Normal University, Beijing

    Wang Yuhuan, Yang Yun & Wu Guoli

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  1. Wang Yuhuan
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  2. Yang Yun
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  3. Wu Guoli
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Wang, Y., Yang, Y. & Wu, G. Comparative study of phosphotyrosyl protein phosphatase of mice normal liver, regenerating liver, and mice H22a hepatoma ascites cell. Chin J Cancer Res 3, 1–6 (1991). https://doi.org/10.1007/BF02997269

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  • DOI: https://doi.org/10.1007/BF02997269

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