Chinese Journal of Cancer Research

, Volume 4, Issue 3, pp 18–23 | Cite as

Isolation and culture of tumor-infiltrating lymphocytes from mouse hepatoma

  • Feng Xuesheng 
  • Li Yiwen 
  • Lin Yunlu 
Basic Investigations


By using enzyme digestion and Ficoll- Hypaque or Percoll discontinuous density methods, we have successfully obtained tumor-infiltrating lymphocytes (TIL) from mouse hepatoma. When analyzing the purity of TIL after separation, it was found that Percoll was more effective than Ficoll (P<0.01). TIL could be activated in the presence of recombinantIL- 2 (rIL-2) and begin to expand after cuituring for 5–7 days, the tumor cells tend to decrease and disappeared after 14 days or so. TIL increased 105-fold over 40 days. Conditioned medium containing supernatant of PHA and rIL-2 stimulated syngeneic spleen cell culture could promote the expansion of TIL.

Key words

mouse hepatoma tumor infiltrating lymphocyte Ficoll- Hypaque Percoll conditioned medium 


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Copyright information

© Chinese Journal Of Cancer Research 1992

Authors and Affiliations

  • Feng Xuesheng 
    • 1
  • Li Yiwen 
    • 2
  • Lin Yunlu 
    • 2
  1. 1.Liver Cancer instituteShanghai Medical UniversityChina
  2. 2.Department of Immunology, School of basic Medical SciencesShanghai Medical UniversityShanghai

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