Isolation and culture of tumor-infiltrating lymphocytes from mouse hepatoma
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By using enzyme digestion and Ficoll- Hypaque or Percoll discontinuous density methods, we have successfully obtained tumor-infiltrating lymphocytes (TIL) from mouse hepatoma. When analyzing the purity of TIL after separation, it was found that Percoll was more effective than Ficoll (P<0.01). TIL could be activated in the presence of recombinantIL- 2 (rIL-2) and begin to expand after cuituring for 5–7 days, the tumor cells tend to decrease and disappeared after 14 days or so. TIL increased 105-fold over 40 days. Conditioned medium containing supernatant of PHA and rIL-2 stimulated syngeneic spleen cell culture could promote the expansion of TIL.
Key wordsmouse hepatoma tumor infiltrating lymphocyte Ficoll- Hypaque Percoll conditioned medium
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