Skip to main content
SpringerLink
Log in

Immobilization of protein on aldehyde-containing gels—II. Activation of pyridine rings with cyanogen bromide

  • Published:
Journal of Solid Phase Biochemistry Aims and scope Submit manuscript

Abstract

Cyanogen bromide was used to convert pyridine rings in polymers to polyaldehyde. By reaction with NH2-containing substances, the rings are rebuilt, resulting in a pyridinium compound. Thus proteins and other NH2-containing substances can be covalently bound. This method provides a new means for a immobilization technique. Pyridine-gels based on polysaccharide and polyacrylamide matrices, as well as pyridine glass beads, were synthesized and used to study the conditions necessary for coupling. Trypsin and — chymotrypsin were used as test substances for immobilization of proteins. Some properties of the bound protein were studied and compared to native enzyme. Some general results on the applicability of these gels for affinity chromatography are also presented.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Porath, J. (1974) Methods Enzymol. 34: 21.

    Google Scholar 

  2. Pittner, F., Miron, T., Pittner, G., andWilchek, M. (1980) J. Solid-Phase Biochem. 5(3): 147, preceding paper.

    Google Scholar 

  3. Miron, T., Carter, W.G., andWilchek, M. (1976) J. Solid-Phase Biochem. 1: 225.

    CAS  Google Scholar 

  4. Winitz, M., andGreenstein, J.P. (1961) Chemistry of the Amino Acids, Wiley, New York, p. 1059.

    Google Scholar 

  5. Weetall, H.H. (1976) Methods Enzymol. 44: 139.

    Google Scholar 

  6. Wilchek, M., andMiron, T. (1974) Mol. Cell. Biochem. 4: 181.

    Article  CAS  Google Scholar 

  7. Levin, Y., Pecht, M., Goldstein, L., andKatchalski, E. (1964) Biochemistry 3:1905.

    Article  CAS  Google Scholar 

  8. Ellmann, G.L. (1959) Arch. Biochem. Biophys. 82: 70.

    Article  Google Scholar 

  9. Critchfield, F. (1963) Organic Functional Group Analysis, Pergamon, New York, p. 78.

    Google Scholar 

  10. Goldstein, L., Levin, Y., andKatchalski, E. (1964) Biochemistry 3: 1913.

    Article  CAS  Google Scholar 

  11. Jacob, S. (1959) Nature 183: 262.

    Article  Google Scholar 

  12. Koenig, W. (1904) J. Prakt. Chem. 69: 105.

    Article  CAS  Google Scholar 

  13. Zincke, Th. (1904) J. Liebig’s Ann. 333: 296.

    Article  Google Scholar 

  14. Marvell, E. N., Caple, G. andShahidi, I. (1970) J. Am. Chem. Soc. 92: 5641.

    Article  CAS  Google Scholar 

  15. Asmus, E., andGarschagen, H. (1953) Z. Anal. Chem. 123: 414.

    Article  Google Scholar 

  16. Kohn, J., andWilchek, M. (1978) Biochem. Biophys. Res. Commun. 84: 7.

    CAS  Google Scholar 

  17. Kudrjavcev, G.I., Vassileva-Sokolova, E.A., Pinskaja, I.S., andVolyn-skaja, T.I. (1965) Faserforschung Textiltechnik 16: 256.

    Google Scholar 

  18. Brooker, L. G. S., White, F. L., Keyes, G. H., Smyth, C.P., andOesper, P.F. (1941) J. Am. Chem. Soc. 63: 3192.

    Article  CAS  Google Scholar 

  19. Moeller, F. (1957) Houben Weyl, Methoden d. Org. Chem. 11/1: 955.

    Google Scholar 

  20. Tsuji, T., Okubo, T., andIse, N. (1976) Nucleic Acids Res., Spec. Publ. 2: 107.

    CAS  Google Scholar 

  21. Cuatrecasas, P. Wilchek, M., andAnfinsen, C.B. (1968) Proc. Natl. Acad. Sci. USA 61:636.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Fritz Pittner

    Present address: Institut fur Allgerneine Biochemie der Universitat Wien, Wahringer Strasse 38, A-1090 Wien, Austria

Authors and Affiliations

  1. Department of Biophysics, The Weizmann Institute of Science Rehovot, Israel

    Fritz Pittner, Talia Miron, Gisela Pittner & Meir Wilchek

Authors
  1. Fritz Pittner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Talia Miron
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gisela Pittner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meir Wilchek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pittner, F., Miron, T., Pittner, G. et al. Immobilization of protein on aldehyde-containing gels—II. Activation of pyridine rings with cyanogen bromide. Journal of Solid Phase Biochemistry 5, 167–180 (1980). https://doi.org/10.1007/BF02994455

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02994455

Keywords

Search

Navigation