Skip to main content
SpringerLink
Log in

Immobilization of protein on aldehyde-containing gels—I. Activation of hydrazide gel with glutaraldehyde

  • Published:
Journal of Solid Phase Biochemistry Aims and scope Submit manuscript

Abstract

Several carriers containing aldehyde groups are described. The carriers were prepared through spacers containing hydrazide groups, followed by glutaraldehyde. Trypsin was coupled to all the carriers, and the influence of temperature, urea, and organic solvents on activity, as well as kinetic parameters of the bound enzyme, were studied. Since all the carriers were prepared similarly, and the same enzyme was coupled to them, similar behaviors were obtained, e.g., two maxima of activity for temperature and organic solvents. Even though all carriers show similar properties for the immobilized enzyme, their preparation and behavior is not always predictable.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zaborski, O. (1973) Immobilized Enzymes, RC Press, Cleveland.

    Google Scholar 

  2. Mosbach, K. (ed.) (1976) Methods Enzymol., Vol. 44, Academic, New York.

    Google Scholar 

  3. Miron, T., Carter, W.G., andWilchek, M. (1976) J. Solid-Phase Biochem. 1: 225.

    CAS  Google Scholar 

  4. Cuatrecasas, P. (1970) J. Biol. Chem. 245: 3059.

    CAS  Google Scholar 

  5. Lamed, R., Levin, Y., andWilchek, M. (1973) Biochim. Biophys. Acta 304: 231.

    CAS  Google Scholar 

  6. Inman, J.K., andDintzis, H.M. (1969) Biochemistry 8: 4074.

    Article  CAS  Google Scholar 

  7. Weetall, H. (1976)In Methods Enzymol., Vol. 44,Mosbach, K. (ed.); Academic,New York, p. 139.

    Google Scholar 

  8. Felix, A.M., andJimenez, M.H. (1973) Anal Bioch. 52: 377.

    Article  CAS  Google Scholar 

  9. Laskowski, M. (1955)In Methods Enzymol., Vol. 11, Academic, New York, p. 8.

    Book  Google Scholar 

  10. Levin, Y., Pecht, M., Goldstein, L., andKatchalski, E. (1964) Biochemistry3:1905.

    Article  CAS  Google Scholar 

  11. Goldstein, L., Levin, Y., andKatchalski, E. (1964) Biochemistry 3: 1914.

    Article  Google Scholar 

  12. Weston, P. D.,andAvrameas, S.(1971)Biochem.Biophys. Res. Commun. 45: 1574.

    Article  CAS  Google Scholar 

  13. Rabjohn, N. (ed.) (1963) Organic Syntheses Coll., Vol. 4, Wiley, New York, p. 819.

    Google Scholar 

Download references

Author information

Author notes

  1. Fritz Pittner

    Present address: Institut fur Allgemeine Biochemie der Universitat Wien, Wahringer Strasse 38, A-1090 Wien, Austria

Authors and Affiliations

  1. Department of Biophysics, The Weizmann Institute of Science Rehovot, Israel

    Fritz Pittner, Talia Miron, Gisela Pittner & Meir Wilchek

Authors
  1. Fritz Pittner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Talia Miron
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gisela Pittner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Meir Wilchek
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pittner, F., Miron, T., Pittner, G. et al. Immobilization of protein on aldehyde-containing gels—I. Activation of hydrazide gel with glutaraldehyde. Journal of Solid Phase Biochemistry 5, 147–166 (1980). https://doi.org/10.1007/BF02994454

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02994454

Keywords

Search

Navigation