Abstract
Conditions for breaking various medically important yeasts using glass beads, 30 ml Corex centrifuge tubes, and a Vortex mixer were determined. From 75–95% ofCandida hyphal cells and all species of yeasts exceptSporothrix schenckii were broken when 10 g of 0.45–0.50 mm glass beads, 50–300 mg of wet cells in 5 ml of buffer, and 90 s of vortexing were employed. Yeasts ofSporothrix schenckii broke more efficiently when 0.25–0.30 mm beads were used.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chaffin, W. L., Barton, R. A., Jacobson, E. L. & Jacobson, M. K., 1979. Nicotinamide adenine dinucleotide metabolism in Candida albicans. J. Bacteriol. 139: 883–888.
Lipinski, C., Ferro A. J. & Mills, D., 1976. Macromolecular synthesis in a mutant of Saccharomyces cerevisiae inhibited by S-adenosylmethionine. Molec. gen. Genet. 144: 301–306.
Ribi, E. & Milner, K. C., 1967. Description of Bacteria and Preparation of Cell Walls, p. 13–28. In Meth. Immunol. Immunochem., vol. 1, C. A. Williams and M. W. Chase (Eds.) Acad. Press, Inc., N.Y.
Richards, W. C. & Takai, S., 1979. A microfuge tube rechnique for disrupting microquantities of fungal spores. Anal. Biochem. 100: 240–243.
Van Etten, J. L. & Freer, S. N., 1978. Simple procedure for disruption of fungal spores. Appl. Environ. Microbiol. 35: 622–623.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hazen, K.C., Cutler, J.E. Optimal conditions for breaking medically important yeasts by an inexpensive and simple method. Mycopathologia 80, 113–116 (1982). https://doi.org/10.1007/BF02993853
Issue Date:
DOI: https://doi.org/10.1007/BF02993853