, Volume 80, Issue 2, pp 113–116 | Cite as

Optimal conditions for breaking medically important yeasts by an inexpensive and simple method

  • K. C. Hazen
  • J. E. Cutler


Conditions for breaking various medically important yeasts using glass beads, 30 ml Corex centrifuge tubes, and a Vortex mixer were determined. From 75–95% ofCandida hyphal cells and all species of yeasts exceptSporothrix schenckii were broken when 10 g of 0.45–0.50 mm glass beads, 50–300 mg of wet cells in 5 ml of buffer, and 90 s of vortexing were employed. Yeasts ofSporothrix schenckii broke more efficiently when 0.25–0.30 mm beads were used.


Candida Albicans Glass Bead Vortex Mixer Candida Tropicalis Hyphal Cell 
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Copyright information

© Dr. W. Junk Publishers 1982

Authors and Affiliations

  • K. C. Hazen
    • 1
  • J. E. Cutler
    • 1
  1. 1.Department of MicrobiologyMontana State UniversityBozemanUSA

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