Biological Trace Element Research

, Volume 26, Issue 1, pp 93–101 | Cite as

Location and quantification of metal ions in enzymes combining polyacrylamide gel electrophoresis and particle-induced X-ray emission

  • Zoltán Szökefalvi-Nagy
  • Csaba Bagyinka
  • István Demeter
  • Kornél L. Kovács
  • Huen Le Quynh
Neutron Activation and Related Methods


A method is presented to identify and determine the relative amounts of protein-bound metal ionsin situ. Proteins or their subunits are directly scanned by a collimated proton beam of 3 MeV energy, and the characteristic X-rays produced are detected. The determination of Fe content of an iron-sulfur protein (HiPiP), as well as the Fe and Ni analysis of the hydrogenese fromThiocapsa roseopersina, have shown the feasibility of this technique.

Index Entries

PIXE particle-induced X-ray emission trace element analysis polyacrylamide gel electrophoresis (PAGE) metalloprotein metalloenzyme bacterial hydrogenase 


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Copyright information

© Humana Press Inc. 1990

Authors and Affiliations

  • Zoltán Szökefalvi-Nagy
    • 1
  • Csaba Bagyinka
    • 2
  • István Demeter
    • 1
  • Kornél L. Kovács
    • 2
  • Huen Le Quynh
    • 2
  1. 1.Central Research Institute for PhysicsHungarian Academy of SciencesBudapest 114Hungary
  2. 2.Institute of Biophysics, Biological Research CenterHungarian Academy of SciencesSzegedHungary

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