Location and quantification of metal ions in enzymes combining polyacrylamide gel electrophoresis and particle-induced X-ray emission
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A method is presented to identify and determine the relative amounts of protein-bound metal ionsin situ. Proteins or their subunits are directly scanned by a collimated proton beam of 3 MeV energy, and the characteristic X-rays produced are detected. The determination of Fe content of an iron-sulfur protein (HiPiP), as well as the Fe and Ni analysis of the hydrogenese fromThiocapsa roseopersina, have shown the feasibility of this technique.
Index EntriesPIXE particle-induced X-ray emission trace element analysis polyacrylamide gel electrophoresis (PAGE) metalloprotein metalloenzyme bacterial hydrogenase
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