Journal of Solid-Phase Biochemistry

, Volume 5, Issue 1, pp 35–44 | Cite as

Organelle sensor: amperometric determination of nadh by immobilized mitochondrial electron transport particles

  • Masuo Aizawa
  • Mitsuo Wada
  • Shuichi Suzuki


A new sensor for NADH was developed by making use of an immobilized subcellular organelle. Mitochondria was used as a model system for assembling an organelle sensor. Mitochondrial electron transport particles (ETP) were prepared from beef heart muscle and entrapped in the membrane formed of agar gel. The membrane-bound ETP was found capable of NADH oxidation:
$$NADH + \tfrac{1}{2}O_2 + H^ + \xrightarrow{{ETP}}NAD^ - + H_2 O$$

The membrane was tightly attached to the surface of an oxygen electrode capable of amperometric detection of O2. The sensor responded to NADH in solution with a resulting electric output. The response was enhanced by the addition of 2,4-dinitrophenol (DNP). NADH was determined in the concentration range 1–300 µM. NADH was alternatively determined for 2 weeks without replacing the ETP-bound membrane.


NADH Electrochemical Device Subcellular Organelle Oxygen Probe Teflon Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc. 1980

Authors and Affiliations

  • Masuo Aizawa
    • 1
  • Mitsuo Wada
    • 1
  • Shuichi Suzuki
    • 1
  1. 1.Research Laboratory of Resources UtilizationTokyo Institute of Technology NagatsutaModiri-ku, Yokohama 227Japan

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