Journal of Solid-Phase Biochemistry

, Volume 5, Issue 1, pp 35–44 | Cite as

Organelle sensor: amperometric determination of nadh by immobilized mitochondrial electron transport particles

  • Masuo Aizawa
  • Mitsuo Wada
  • Shuichi Suzuki


A new sensor for NADH was developed by making use of an immobilized subcellular organelle. Mitochondria was used as a model system for assembling an organelle sensor. Mitochondrial electron transport particles (ETP) were prepared from beef heart muscle and entrapped in the membrane formed of agar gel. The membrane-bound ETP was found capable of NADH oxidation:
$$NADH + \tfrac{1}{2}O_2 + H^ + \xrightarrow{{ETP}}NAD^ - + H_2 O$$

The membrane was tightly attached to the surface of an oxygen electrode capable of amperometric detection of O2. The sensor responded to NADH in solution with a resulting electric output. The response was enhanced by the addition of 2,4-dinitrophenol (DNP). NADH was determined in the concentration range 1–300 µM. NADH was alternatively determined for 2 weeks without replacing the ETP-bound membrane.


NADH Electrochemical Device Subcellular Organelle Oxygen Probe Teflon Membrane 


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Copyright information

© Humana Press Inc. 1980

Authors and Affiliations

  • Masuo Aizawa
    • 1
  • Mitsuo Wada
    • 1
  • Shuichi Suzuki
    • 1
  1. 1.Research Laboratory of Resources UtilizationTokyo Institute of Technology NagatsutaModiri-ku, Yokohama 227Japan

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