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Efficiency of two enzymes immobilized to the same surface and acting in sequence

II. Relative efficiencies of a two-enzyme sequential reacti in either the soluble or the immobilized state

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Abstract

Phosphoglucomutase and glucose-6-phosphate dehydrogenase were immobilized to striazine trichloride activated cellulose to determine if perparations having two enzymes immobilized to the same surface could carry out the sequential reaction, glucose-1-P to glucose-6-P, glucose-6-P to 6-phosphogluconate more efficiently than preparations in which the enzymes were immobilized to separate particles or free in solution. The results of experiments examining the effect of dilution on the sequential rate indicate greater efficiency by enzymes immobilized to the same surface.

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References

  1. Lynen, F (1967) Biochem. J. 101: 381–400.

    Google Scholar 

  2. Welch, R. L andGaertner, F. H (1975) Proc. Natl. Acad. Sci. U.S.A. 72:4218–4222.

    Article  CAS  Google Scholar 

  3. Matchett, W. H (1974) J. Biol. Chem. 249: 4041–4049.

    CAS  Google Scholar 

  4. Lue, P. F andKaplan, J. G (1970) Biochim. Biophys. Acta 220: 365–372.

    CAS  Google Scholar 

  5. Gaertner, F. H Ericson, M. C andDeMoss, J. A (1970) J. Biol. Chem. 245:585–600.

    Google Scholar 

  6. Keilin, D andHartee, E. F (1940), Roy. Soc. London, Proc. 129: 277–306.

    Article  CAS  Google Scholar 

  7. Weiss, R. L andDavis, R. H (1973) J. Biol. Chem. 248: 5403–5408.

    CAS  Google Scholar 

  8. Zalokar, M (1960) Exp. Cell Res. 19: 114.

    Article  CAS  Google Scholar 

  9. Lloyd-Davies, K. A Michell, R. H andColeman, R (1972) Biochem. J. 127: 357–368.

    CAS  Google Scholar 

  10. Katchalski, E Silman, I andGoldman, R (1971)In Methods in Enzymology, Vol. 34,Nord, F. F (ed.), Interscience Publishers, New York, pp. 445–536.

    Google Scholar 

  11. Davis, R. H (1967)In Organizational BiosynthesisVogel, H. J Lampen, J. O andBryson, V (eds.), Academic Press, New York, pp. 303–322.

    Google Scholar 

  12. Davis, R. H (1972) Science 24: 835–840.

    Article  Google Scholar 

  13. Shimizu, S. Y andLenhoff, H. M (1979) J. Solid Phase Biochem. 4: 75–94.

    CAS  Google Scholar 

  14. Kiritani, K Narise, S Berquist, A andWagner, R. P (1965) Biochim. Biophys. Acta 100: 432–443.

    CAS  Google Scholar 

  15. Wagner, R. P Bergquist, A Brotzman, B andEakin, E. A (1967)In Organizational Biosynthesis,Vogel, J. H Lampen, J. O andBryson, U (eds.), Academic Press, New York, pp. 267–293.

    Google Scholar 

  16. Goldman, R Goldstein, L andKatchalski, E (1971)In Biochemical Aspects of Reactions on Solid Supports,Stark, G. R (ed.), Academic Press, New York, pp. 1–78.

    Google Scholar 

  17. Nernst, W. A (1904) Z. Phys. Chem. 47: 52.

    CAS  Google Scholar 

  18. Dorfner, K (1972)In Ion Exchangers-Properties and Applications,Coers, A. F (ed.), Science Publications, Inc., Ann Arbor, MI, pp. 250–255.

    Google Scholar 

  19. Boyd, G. E. , Admonson, A. W., and Meyer, Jr., L. S. (1947) J. Am. Chem. Soc, 2836–2848.

  20. Ginzburg, B. A andKatchalski, A (1963) J. Gen Physiol. 47: 403–418.

    Article  CAS  Google Scholar 

  21. Helfferich, F (1962) Ion Exchange, McGraw-Hill, New York, pp 253–258.

    Google Scholar 

  22. Thiele, E.W (1939) Ind. Eng. Chem. 31: 916–920.

    Article  CAS  Google Scholar 

  23. Reichenberg, D (1953) J. Am. Chem. Soc. 75: 589.

    Article  CAS  Google Scholar 

  24. Goldman, R andKatchalski, E (1971) J. Theor. Biol. 32: 243–257.

    Article  CAS  Google Scholar 

  25. Bouin, J. C Atallah, M. T andHultin, H. O (1976) Biotech. Bioeng. 18: 179–187.

    Article  CAS  Google Scholar 

  26. Campbell, J andChang, T. M. S (1975) Biochim. Biophys. Acta. 397:101–109.

    CAS  Google Scholar 

  27. Hervagault, J. F Joly, G andThomas, D (1975) Eur. J. Biochem. 51, 19–23.

    Article  CAS  Google Scholar 

  28. Ho, S. P andKostin, M. D (1974) J. Chem. Phys. 61: 918–920.

    Article  CAS  Google Scholar 

  29. Hultin, H. O (1974) J. Food Sci. 39: 652.

    Article  Google Scholar 

  30. Martensson, K (1974) Biotech. Bioeng. 16: 567–577.ba]

    Article  CAS  Google Scholar 

  31. Martensson, K (1974) Biotech. Bioeng. 16, 579–591.

    Article  CAS  Google Scholar 

  32. Markey, P. E Greenfield, P. F andKittreu, J. R (1975) Biotech. Bioeng. 17:285–289.

    Article  CAS  Google Scholar 

  33. Mattiasson, B andMosbach, K (1971) Biochim. Biophys. Acta 235: 253–257.

    CAS  Google Scholar 

  34. Mosbach, K andMattiasson, B (1970) Acta Chem. Scand. 24: 2093–2100.

    Article  CAS  Google Scholar 

  35. Srere, P. A andMosbach, K (1974) Ann. Rev. Micro. 28: 61–83.

    Article  CAS  Google Scholar 

  36. Shimizu, S. Y andLenhoff, H. M (1978)In Enzyme Engineering, Vol. 3Pye, E. K andWeetal, H. H (eds.), Plenum Press, New York, pp 155–161.

    Google Scholar 

  37. Lenhoff, H. M Shimizu, S. Y andSmith, N. L (1977),In Biomedical Applications of Immobilized Enzymes and Proteins, Vol. 2,Chang, T. M. S (ed.), Plenum Press, New York, pp. 271–279.

    Google Scholar 

  38. Shimizu, S. Y andLenhoff, H. M (1979) J. Solid Phase Biochem. 4:109–122.

    CAS  Google Scholar 

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Author notes

  1. Stanley Y. Shimizu

    Present address: Department of Biochemistry and Pharmacology, Tufts University, School of Medicine, 136 Harrison Ave, 02111, Boston, Massachusetts

Authors and Affiliations

  1. Department of Developmental and Cell Biology and Molecular Biology and Biochemistry, University of California, 92717, Irvine, California

    Stanley Y. Shimizu & Howard M. Lenhoff

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  1. Stanley Y. Shimizu
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  2. Howard M. Lenhoff
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Shimizu, S.Y., Lenhoff, H.M. Efficiency of two enzymes immobilized to the same surface and acting in sequence. Journal of Solid Phase Biochemistry 4, 95–107 (1979). https://doi.org/10.1007/BF02991864

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  • DOI: https://doi.org/10.1007/BF02991864

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