Efficiency of two enzymes immobilized to the same surface and acting in sequence

II. Relative efficiencies of a two-enzyme sequential reacti in either the soluble or the immobilized state
  • Stanley Y. Shimizu
  • Howard M. Lenhoff


Phosphoglucomutase and glucose-6-phosphate dehydrogenase were immobilized to striazine trichloride activated cellulose to determine if perparations having two enzymes immobilized to the same surface could carry out the sequential reaction, glucose-1-P to glucose-6-P, glucose-6-P to 6-phosphogluconate more efficiently than preparations in which the enzymes were immobilized to separate particles or free in solution. The results of experiments examining the effect of dilution on the sequential rate indicate greater efficiency by enzymes immobilized to the same surface.


Enzyme Dehydrogenase Activity Immobilize Enzyme Dehy Frit Glass 
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Copyright information

© Humana Press Inc. 1979

Authors and Affiliations

  • Stanley Y. Shimizu
    • 1
  • Howard M. Lenhoff
    • 1
  1. 1.Department of Developmental and Cell Biology and Molecular Biology and BiochemistryUniversity of CaliforniaIrvine

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