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Preparation and properties of immobilized diphtheria toxin

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Abstract

Commercially available diphtheria toxin was immobilized on nylon stocking material by a covalent activation of the polymeric material. Toxin was bound both directly to nylon as well as to nylon-polyethyleneimine copolymer. The immobilized toxin retained some of its catalytic activity as evidenced by ADP-ribosylation of elongation factor II. Kinetics of this reaction are reported. After repeated use for several times the immobilized toxin retains 75% of its activity.

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Authors and Affiliations

  1. Abteilung Chemie, Max-Planck-Institut für Experimented Medizin, D-34, Göttingen, West Germany

    P. V. Sundaram

  2. Biofizik Kürsüsü, Istanbul Tip Fakültesi, Istanbul Universitesi, Capa, Istanbul, Turkey

    E. Bermek

Authors
  1. P. V. Sundaram
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  2. E. Bermek
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Sundaram, P.V., Bermek, E. Preparation and properties of immobilized diphtheria toxin. Journal of Solid Phase Biochemistry 3, 263–270 (1978). https://doi.org/10.1007/BF02991852

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  • DOI: https://doi.org/10.1007/BF02991852

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