Preparation of immobilized tannins for protein adsorption
- 77 Downloads
Preparation and adsorption specificity of tannins immobilized by covalent binding on water-insoluble matrices were investigated. Immobilized tannins were prepared by condensing cyanogen bromide activated tannins with aminohexyl derivatives of several kinds of matrices. The most suitable matrix for the immobilization of tannin was alkali-treated cellulose powder. The concentration of sodium hydroxide solution for alkali treatment influenced the adsorption capacity of immobilized tannin for a protein, but temperature and time for alkali treatment did not. Immobilized tannins having spacers of long chain length exhibited high adsorption capacity for a protein. Chinese gallotannin was the most favorable ligand for protein adsorption. The immobilization of tannin on aminohexyl matrices was also possible by using epichlorohydrin instead of cyanogen bromide. The maximum adsorption capacity of the immobilized tannin for a protein was about 50 mg/ml of the absorbent. Immobilized tannin adsorbed proteins specifically but did not absorb low molecular weight compounds.
KeywordsAdsorption Capacity Tannin High Adsorption Capacity Alkali Treatment Epichlorohydrin
Unable to display preview. Download preview PDF.
- 1.Grant, R. A. (1974) Process Biochemistry 9: 11–14.Google Scholar
- 2.Messing, R. A. (1974) Brewers Digest 46: 60–63.Google Scholar
- 3.Atkinson, A. (1973) Process Biochemistry 8: 9–12.Google Scholar
- 4.Baum, G., andLynn, M. (1973) Process Biochemistry 10: 14–17.Google Scholar
- 6.Cuatrecasas, P. (1970) J. Biol. Chem. (1970) 245: 3059–3065Google Scholar
- 10.Hoare, D. G., andKoshland, D. E. (1967) J. Biol. Chem. 242: 2447–2453.Google Scholar
- 11.Masaharu, K. (1949) Yakugaka Kenkyu 21: 129–132.Google Scholar
- 12.Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. (1951) J. Biol. Chem. 193: 265–275.Google Scholar
- 14.Somogi, M. (1945) J. Biol. Chem. 160: 61–68.Google Scholar
- 19.Watanabe, T.,Fujimura, M.,Mori, T.,Tosa, T., andChibata, I. (in press) J. Appl. Biochem.Google Scholar