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Journal of Solid Phase Biochemistry

, Volume 3, Issue 3, pp 161–174 | Cite as

Preparation of immobilized tannins for protein adsorption

  • Taizo Watanabe
  • Yuhsi Matuo
  • Takao Mori
  • Ryujiro Sano
  • Tetsuya Tosa
  • Ichiro Chibata
Article
  • 83 Downloads

Abstract

Preparation and adsorption specificity of tannins immobilized by covalent binding on water-insoluble matrices were investigated. Immobilized tannins were prepared by condensing cyanogen bromide activated tannins with aminohexyl derivatives of several kinds of matrices. The most suitable matrix for the immobilization of tannin was alkali-treated cellulose powder. The concentration of sodium hydroxide solution for alkali treatment influenced the adsorption capacity of immobilized tannin for a protein, but temperature and time for alkali treatment did not. Immobilized tannins having spacers of long chain length exhibited high adsorption capacity for a protein. Chinese gallotannin was the most favorable ligand for protein adsorption. The immobilization of tannin on aminohexyl matrices was also possible by using epichlorohydrin instead of cyanogen bromide. The maximum adsorption capacity of the immobilized tannin for a protein was about 50 mg/ml of the absorbent. Immobilized tannin adsorbed proteins specifically but did not absorb low molecular weight compounds.

Keywords

Adsorption Capacity Tannin High Adsorption Capacity Alkali Treatment Epichlorohydrin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1979

Authors and Affiliations

  • Taizo Watanabe
    • 1
  • Yuhsi Matuo
    • 1
  • Takao Mori
    • 1
  • Ryujiro Sano
    • 1
  • Tetsuya Tosa
    • 1
  • Ichiro Chibata
    • 1
  1. 1.Department of BiochemistryResearch Laboratory of Applied Biochemistry Tanabe Seiyaku Co., Ltd.OsakaJapan

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