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Journal of Solid Phase Biochemistry

, Volume 3, Issue 2, pp 151–160 | Cite as

Characterization of an immobilized antibody-enzyme complex

  • Hugh C. Mcdonald
  • Howard h. weetall
  • Paula Simon
  • Milton M. Takeguchi
  • Gerald Odstrchel
Article
  • 17 Downloads

Abstract

Antiserum specific for propanediol dehydrogenase, an enzyme found inNeisseria gonorrhoeae cells, has been immobilized to glass. When mixed withN gonorrhoeae cell lysates, the immobilized antibody (IMA) binds the enzyme. Over 70% of the calculated adsorbed activity can be recovered from the immobilized antibody-enzyme (IMA-E) complex. When mixed with bacterial lysates prepared from different organisms having propanediol dehydrogenase-like activity, the IMA specifically adsorbed the enzyme from theN gonorrhoeae lysate. IMA-E complexes have been prepared and their kinetic, temperature and chemical stability, and antigenic properties investigated. These studies demonstrated the feasibility of using an immobilized antibody in the detection of the propanediol dehydrogenase enzyme.

Keywords

Propanediol Free Enzyme Neisseria Gonorrhoeae Guanidine Hydrochloride Bacterial Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Weetall, H. H.,Takeguchi, M. M.,McDonald, H. C.,Detar, C. C.,Simon, P. A.,Livsey, K. A.,Odstrchel, G., andKaplan, N. O., in preparation.Google Scholar
  2. 2.
    McDonald, H. C,Simon, P.,Weetall, H. H.,Takeguchi, M. M., andOdstrchel, G., in preparation.Google Scholar
  3. 3.
    Weetall, H. H. andFilbert, A. M. (1974).In Methods in Enzymology34B Jakoby, W. B., andWilcheck, W. (eds.), Academic Press, New York, pp. 59–72.Google Scholar

Copyright information

© Humana Press Inc. 1979

Authors and Affiliations

  • Hugh C. Mcdonald
    • 1
  • Howard h. weetall
    • 1
  • Paula Simon
    • 1
  • Milton M. Takeguchi
    • 1
  • Gerald Odstrchel
    • 1
  1. 1.Corning Glass WorksResearch & Development LaboratoriesCorning, New York

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