Abstract
The posterior silk glands of the silkworm, (itBombyx mori,) were immobilized in acrylamide gel. This immobilized organ produced silk protein in the presence of amino acids and energy sources. Amino acids began to be incorporated into the immobilized silk glands 2.5 h after incubation, and the incorporation continued for more than 20 h. The apparent lifetime of the posterior silk gland was remarkably elongated by the immobilization.
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References
Weetall, H. H., andSuzuki, S. (eds.) (1975) Immobilized Enzyme Technology, Plenum, New York.
Mosbach, K. (ed.) (1976) Methods in Enzymology, Vol. 44, Academic, New York.
Pye, K., andWingard, L. B., Jr. (eds.) (1978) Enzyme Engineering, Vols. I-IV, Plenum, New York.
Chibata, I. (1978) Immobilized Enzymes, Academic Press Center, Tokyo.
Tashiro, Y., Morimoto, T., Matsuura, S., andNagata, S. (1968) J. Cell Biol. 38: 574.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., andRandall, R. J. (1951) J. Biol. Chem. 193: 265.
Iizuka, M., andKobayashi, S. (1978) Kagaku To Seibutsu 16: 686.
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Ikariyama, Y., Aizawa, M. & Suzuki, S. Silk protein production by the immobilized silk gland. Journal of Solid-Phase Biochemistry 4, 69–73 (1979). https://doi.org/10.1007/BF02991809
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DOI: https://doi.org/10.1007/BF02991809