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Coupling of effector molecules to solid supports

Development of an alternative to the cyanogen bromide activation of polysaccharides
  • T. C. J. Gribnau
  • G. I. Tesser
  • R. J. F. Nivard
Article
  • 24 Downloads

Abstract

In view of the limited stability of the isourea bond, formed in ligand coupling to CNBractivated polysaccharides, an alternative to this current activation method has been developed. 2,4,6-Trifluoro-5-chloropyrimidine (FCP), known as a reactive group in reactive dyes, was used to activate Sepharose. Under appropriate conditions a thermally stable product with unimpaired beaded structure was obtained, which was reactive toward amines and mercaptans. Coupling with hexamethylenediamine, aniline, and ethanethiol, respectively, yielded an incorporation of 0.2-2.7, 0.9-1.7, and 1.1 mmol ligand/g dry agarose. The stability of immobilized ligands based on FCP-Sepharose between pH 4 and 8 was about 200 times higher as compared to products originating from CNBr-Sepharose; ligand leakage was only 0.5 x 10p-3%/h. The possibility of obtaining a high degree of substitution is a further advantage of the FCP activation. In addition, the FCP-activated Sepharose can be stored in the wet state at 4°C without substantial decrease in coupling capacity. The FCP analogs 2,4,5,6-tetrachloro- and 2,4,5,6-tetrafluoropyrimidine, and other polymers (cellulose, Sephadex, aminomethylpolystyrene) appeared to be applicable also.

Keywords

Effector Molecule Mercaptan Fluorine Content Ethanethiol Cyanuric Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1978

Authors and Affiliations

  • T. C. J. Gribnau
    • 1
  • G. I. Tesser
    • 1
  • R. J. F. Nivard
    • 1
  1. 1.Department of Organic ChemistryCatholic UniversityToernooiveldThe Netherlands

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